Application of cyclic dipeptide C7 in Phellinus igniarius in resisting avian influenza H5N1 virus
A technology of Phylophorus spp. and cyclic dipeptides, applied in antiviral agents, applications, medical preparations containing active ingredients, etc.
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example 1
[0037] The crude extract of Phylophorus chrysogenum is prepared by the following method:
[0038] (1) The fermentation medium formula is in grams / 100 milliliters:
[0039] Corn Starch 1% Glucose 1%
[0040] Peptone 0.1% Yeast Extract 0.1%
[0041] Magnesium sulfate 0.1% Potassium dihydrogen phosphate 0.01%
[0042] (2) Inoculate the Phyloporus spp. strains into the Erlenmeyer flask with the liquid medium in a conventional method, with a temperature of 25° C., a shaking flask rotation speed of 110 r / min, and pH 7 conditions, and vibrate for 7 days; cultivate When the pH value drops to 3, inoculate the seeds in the shake flask into the culture solution of a 50L fermenter, stir at a temperature of 25°C, a fermenter pressure of 0.1 kg / cm2, a pH of 3, and an air flow of 0.5-1.1vvm. Under the condition of a speed of 100 r / min, after culturing for 7 days, the crude extract of Pheronotus can be prepared by using the whole liquid fermented with the mycelia of Ph.
[0043] (3) Take ...
example 2
[0053] The crude extract of Phylophorus chrysogenum is prepared by the following method:
[0054] (1) The fermentation medium formula is in grams / 100 milliliters:
[0055] Corn Starch 3% Glucose 2%
[0056] Peptone 0.5% Yeast Extract 0.5%
[0057] Magnesium sulfate 0.5% Potassium dihydrogen phosphate 0.05%
[0058] (2) Inoculate the Phylophorus spp. strain into the Erlenmeyer flask with liquid medium in a conventional way, with a temperature of 30° C., a shaker flask rotation speed of 180 r / min, and pH 6 conditions, and vibrate for 15 days; during the cultivation When the pH value drops to 2.5, inoculate the seeds in the shake flask into the culture solution of a 50L fermenter, with a temperature of 30°C, a fermenter pressure of 0.2 kg / cm2, pH 3, an air flow of 0.5-1.1vvm, and a stirring speed Under the condition of 180 rev / min, after culturing for 15 days, the crude extract of Pheronotus can be prepared by using the fermented whole solution of Phyloporea mycelium.
[0059...
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