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Method for creating new germplasms of macadimia nuts

A new germplasm and nut technology, applied in the direction of botanical equipment and methods, application, plant gene improvement, etc., can solve problems that have not been reported, achieve large economic benefits, and shorten the breeding cycle of varieties

Inactive Publication Date: 2014-05-21
YUNNAN INST OF TROPICAL CROPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 60 Since Co-γ rays were applied to the field of plant mutation breeding in the last century, they have been used in the breeding of many staple crops and some economic crops such as fruit trees, vegetables and flowers, and have achieved remarkable results. However, due to the special nature of macadamia structure, will 60 The application of Co-γ-ray irradiation to germinate seeds of macadamia nuts for germplasm innovation has not been reported at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Collect mature seeds of macadamia main varieties D4, O.C, HAES900, A16, place them in a sterilized moist sand bed to accelerate germination immediately after artificial peeling, and screen the fruits whose shells are cracked by more than 1mm, but the bud points have not yet protruded. The germination of the shell proceeds from the seed 60 co -γ For radiation mutagenesis, the irradiation doses were 10 Gy, 40 Gy, 70 Gy, and 100 Gy, respectively, and the dose rate was 10 Gy / h. The germinated seeds after irradiation were placed in the sterilized moist sand bed to accelerate germination, and the investigation on the germination rate found that: 60 co -γ Radiation irradiation had a significant inhibitory effect on the germination rate of germinated seeds. With the increase of irradiation dose, the germination rate of seeds slowed down significantly; and with the increase of irradiation dose, the germination rate also decreased significantly. Among them: the opti...

Embodiment 2

[0021] Example 2: Similar to Example 1, the germinated seeds of the above varieties were dosed with 25 Gy, 55 Gy, 85 Gy, 115 Gy, 130 Gy 60 co -γ Rays were irradiated, and the germination and morphological characteristics of germinated seeds after irradiation were investigated. It was found that varieties HAES900 and variety A16 were treated with a dose of 115 Gy 60 co -γ All of them were killed after being irradiated with ray, and the varieties D4 and O.C were treated with a dose of 130 Gy 60 co -γ Immediately be all lethal after ray irradiation; The impact on seed germination speed and germination rate is the same as in Example 1; About 30% of the seedlings have a significant increase in the stem diameter described in Example 1, a significant decrease in stem height, and a short plant type The main root disappears and the fibrous root system is developed, the first round of true leaves is deformed or disappears, and the second round of true leaves changes in shape; about...

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Abstract

The invention discloses a method for creating new germplasms of macadimia nuts, relating to a method for creating new germplasms of macadimia nuts by irradiating gemmiferous seeds of macadimia nuts through 60 Co-gamma rays. The method disclosed by the invention comprises the following steps: seeding mature seeds of fresh macadimia nuts in a sand bed that the width from germination accelerating to cracking is more than 1 mm; discarding seeds, bud points of which protrude shells, and uncracked seeds; irradiating gemmiferous seeds, the cracking degrees of which are proper, by using 60 Co-gamma rays, the dosage of which is 10-130 Gy; after irradiating, continuously seeding into the sand bed to continuously sprout and grow; observing morphological changes of mutagenesis seedlings, such as plant height, stem thickness and blade distortion; and obtaining new germplasms of macadimia nuts, and fixedly planting in a land. The method disclosed by the invention is capable of rapidly and effectively creating a large number of new germplasms of macadimia nuts, overcoming the germplasm resource shortage actuality of macadimia nuts in China and expanding the germplasm resources of macadimia nuts.

Description

technical field [0001] The present invention relates to a kind of germplasm creating method, specifically a method through 60 A method of germinating seeds of macadamia nuts irradiated with Co-γ rays to create new germplasm of macadamia nuts. technical background [0002] Macadamia spp. is an evergreen tree of the genus Macadamia F.Muell of the family Proteaceae. It is native to the subtropical rainforests of southeastern Queensland and northern New South Wales, Australia. It was introduced in the late 19th century. To Hawaii, USA, and began large-scale commercial planting in Hawaii after 1948. Since the late 1970s, my country has introduced and tried to plant macadamia nuts. It is currently distributed in Yunnan, Guangdong, Guangxi, Hainan and other hot-spot provinces, but the main commercial plantation is still in Yunnan. [0003] Due to the relatively short time for the introduction and cultivation of macadamia nuts in China, the current production mainly uses the bred...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06
Inventor 柳觐倪书邦贺熙勇孔广红
Owner YUNNAN INST OF TROPICAL CROPS
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