Method for creating new germplasms of macadimia nuts
A new germplasm and nut technology, applied in the direction of botanical equipment and methods, application, plant gene improvement, etc., can solve problems that have not been reported, achieve large economic benefits, and shorten the breeding cycle of varieties
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Embodiment 1
[0020] Example 1: Collect mature seeds of macadamia main varieties D4, O.C, HAES900, A16, place them in a sterilized moist sand bed to accelerate germination immediately after artificial peeling, and screen the fruits whose shells are cracked by more than 1mm, but the bud points have not yet protruded. The germination of the shell proceeds from the seed 60 co -γ For radiation mutagenesis, the irradiation doses were 10 Gy, 40 Gy, 70 Gy, and 100 Gy, respectively, and the dose rate was 10 Gy / h. The germinated seeds after irradiation were placed in the sterilized moist sand bed to accelerate germination, and the investigation on the germination rate found that: 60 co -γ Radiation irradiation had a significant inhibitory effect on the germination rate of germinated seeds. With the increase of irradiation dose, the germination rate of seeds slowed down significantly; and with the increase of irradiation dose, the germination rate also decreased significantly. Among them: the opti...
Embodiment 2
[0021] Example 2: Similar to Example 1, the germinated seeds of the above varieties were dosed with 25 Gy, 55 Gy, 85 Gy, 115 Gy, 130 Gy 60 co -γ Rays were irradiated, and the germination and morphological characteristics of germinated seeds after irradiation were investigated. It was found that varieties HAES900 and variety A16 were treated with a dose of 115 Gy 60 co -γ All of them were killed after being irradiated with ray, and the varieties D4 and O.C were treated with a dose of 130 Gy 60 co -γ Immediately be all lethal after ray irradiation; The impact on seed germination speed and germination rate is the same as in Example 1; About 30% of the seedlings have a significant increase in the stem diameter described in Example 1, a significant decrease in stem height, and a short plant type The main root disappears and the fibrous root system is developed, the first round of true leaves is deformed or disappears, and the second round of true leaves changes in shape; about...
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