Method for inhibiting late endothelial progenitor cell function

An endothelial progenitor cell and functional technology, applied in the field of simulating the prevention of infection or permanent adhesion after surgery, can solve the problem of short activity retention time of anti-adhesion membrane materials, achieve excellent physical and chemical properties, and prevent permanent adhesion. The effect of generating and reducing the formation of new blood vessels

Inactive Publication Date: 2014-05-14
WEIFANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The disadvantage of chitosan anti-adhesion film currently used clinically is that the active retention time of chitosan anti-adhesion film material is relatively short

Method used

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  • Method for inhibiting late endothelial progenitor cell function
  • Method for inhibiting late endothelial progenitor cell function
  • Method for inhibiting late endothelial progenitor cell function

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Experimental program
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Embodiment 1

[0036] ① Isolation, culture and identification of rat bone marrow EPCs

[0037] Rat bone marrow mononuclear cells were separated by density gradient centrifugation, and the cells were resuspended in complete EGM-2 medium, inoculated into culture flasks primed with FN, and placed at 37°C containing 5% CO 2 (volume percent) in the incubator. The culture medium was changed after 4 days, and the medium was changed every 3 days thereafter. When the cells grew to about 80%, they were subcultured with 0.25% (weight percent) trypsin at a ratio of 1:1. The third-generation EPCs, that is, the late EPCs, were used as target cells. The cells were stained with Dil-ac-LDL and FITC-UEA-1, and the double staining was positive. Flow cytometry was used to detect VEGFR2, vWF, CD31, and VE-Cadherin, etc. Expression of endothelial cell markers.

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Abstract

The invention discloses a method for inhibiting late endothelial progenitor cell function. According to the method, a polylactic acid antiadhnesive membrane is adopted. Compared with the prior art, the method disclosed by the invention can be used for inhibiting proliferation, adhesion, migration or angiogenesis of late endothelial progenitor cells, and reducing neovascularization, so as to prevent infection or postoperative permanent adhesion.

Description

technical field [0001] The invention relates to a method for inhibiting the function of late endothelial progenitor cells, a method for reducing the formation of new blood vessels and a method for simulating the prevention of permanent adhesion after infection or operation. Background technique [0002] Infection or surgery can easily cause adhesion, and the process of adhesion is relatively complicated. In addition to being affected by the disorder of fibrinolytic mechanism, various cellular factors and growth factors also have a certain impact on the formation of adhesion. Studies have shown that if postoperative adhesions fail to dissolve for 3 days, fibroblasts will proliferate and new capillaries will grow into them, forming permanent fibrous adhesions between the tissues in contact with each other. [0003] Existing studies on adhesion basically focus on fibroblasts, but the formation of new blood vessels is an important process in the formation of permanent adhesions....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/765A61P41/00C12N5/0789
Inventor 成敏官秀梅李鑫
Owner WEIFANG MEDICAL UNIV
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