Method for improving output of alpha-arbutin

Abstract

The invention relates to a preparation method for synthesizing alpha-arbutin through immobilizing hydroquinone by a macro-porous adsorption resin and carrying out fermentation catalysis of the immobilized hydroquinone and disaccharide by Xanthomonas campestris having a preservation number of CGMCC NO.1243. The method comprises the steps of immobilized hydroquinone preparation, microbial fermentation, reaction condition optimization, and product separation and extraction. The alpha-arbutin output of the method is 20-235g each liter of a broth, and is 14 times higher than the output of alpha-arbutin obtained by directly using hydroquinone as a reactant, the hydroquinone conversion rate of the method reaches 90.5-95.2%, and the purity of the above obtained separated and purified product can reach above 99.5%. The immobilization of the hydroquinone improves the tolerance of a thallus to hydroquinone, simplifies the subsequent separating and purifying technology of the product, and reduces the phenol-containing wastewater emission of the above system; and the method has the advantages of high output, simple production technology, low production cost, environmental protection, and substantial reduction of the alpha-arbutin production cost, and lays a foundation for the large-scale low-cost production of alpha-arbutin.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the use of macroporous adsorption resin to immobilize hydroquinone, and to synthesize α-bear from xanthomonas campestris (Xanthomonas campestris) CGMCC NO.1243 fermentation catalyzed immobilized hydroquinone and disaccharides The preparation method of fruit glycosides. Background technique [0002] α-Arbutin has the activity of significantly inhibiting tyrosinase, can reduce the deposition of tyrosinase in the skin, and has the effects of bleaching, preventing discoloration and freckle on the skin. When comparing the whitening effect of α-arbutin and its isomer β-arbutin, Kazuhisa Sugimotod et al. found that the whitening effect of α-arbutin was β-arbutin through experiments on human melanoma cells. more than 10 times. At present, α-Arbutin is an efficient and safe whitening agent recognized internationally and is being vigorously promoted. It is an ideal additive ingredient for whit...

AI Technical Summary

Problems solved by technology

[0006] 1. The tolerance of the catalyst (bacteria or enzyme) to hydroquinone is not high, resulting in a low yield of α-arbutin;

[0007] 2. The conversion rate of α-arbutin is not high, resulting in a low utilization rate of raw materials;

[0008] 3. The subsequent separation and purification process of the product α-arbutin is complicated, and the equipment investment and operating costs are relatively large;

[0009] 4. During the production process, waste water containing hydroquinone is produced, polluting the environment