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Alleles for regulating and controlling two types of main stem table fur types of upland cotton, and single stranded conformational polymorphism (SSCP) molecular marker for identification thereof

An allele and molecular marker technology, applied in genetic engineering, plant genetic improvement, and microbial determination/inspection, etc., can solve the problem that the influence and function of GhHD-1 gene are not explained.

Inactive Publication Date: 2014-04-30
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology

However, the above-mentioned studies did not explain the influence and effect of GhHD-1 gene on stem epidermis or fiber quality.

Method used

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  • Alleles for regulating and controlling two types of main stem table fur types of upland cotton, and single stranded conformational polymorphism (SSCP) molecular marker for identification thereof
  • Alleles for regulating and controlling two types of main stem table fur types of upland cotton, and single stranded conformational polymorphism (SSCP) molecular marker for identification thereof
  • Alleles for regulating and controlling two types of main stem table fur types of upland cotton, and single stranded conformational polymorphism (SSCP) molecular marker for identification thereof

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Embodiment Construction

[0024] The technical solutions of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0025] 1. DNA sequence differences of GhHD1 gene in different upland cotton

[0026]Our laboratory designed specific primers for the HD1 gene (At-GhHD1) of the upland cotton A genome, and used the primers to amplify the full-length sequence of the GhHD1 gene from different (50) upland cotton varieties. Sequencing analysis showed that there were two types of The DNA sequence is represented by type A1 and type A2. Sequence comparison reveals that there is a difference of 8 bases between type A1 and type A2, of which 5 bases are in the exon (Appendix 1), making them have 5 amino acid differences ( figure 1 ). Analysis of expression in different tissues showed that GhHD1 gene was predominantly expressed in stems.

[0027] 2. Observation and analysis of upland cotton stalk epidermis

[0028] The phenotype of ...

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Abstract

The invention discloses alleles for regulating and controlling two types of main stem table fur types of upland cotton, and a single stranded conformational polymorphism (SSCP) molecular marker for identification thereof. On the research basis of defining that an HDI gene in a homeobox-homeodomain-leucine zipper protein family IV is a key gene of affecting table fur generation of the upland cotton, the alleles GhHD1a and b for regulating and controlling regeneration of two types of stem table furs of the upland cotton are identified for the first time; a molecular marker for screening the two alleles by using the SSCP technology is invented. The molecular marker can rapidly identify two kinds of table fur types. The alleles prove that two genetypes of the HD1 have close relationship to two table fur types, a foundation is established for researching the molecular mechanism of generating table furs and fibers, the deoxyribonucleic acid (DNA) molecular marker capable of directly identifying the table fur type of the upland cotton in a laboratory is developed, and a rapid technology is provided for rapidly breeding different fur types of new cotton varieties.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an allele that regulates two types of main stalk hair types of upland cotton and an SSCP molecular marker used for its identification. Background technique [0002] Fiber is the main product harvested in cotton production and is the main raw material for the textile industry. High-yield and high-quality fiber is the ultimate goal of cotton breeding. Cotton fiber can be divided into two categories according to length: fuzzy fiber (<5mm) and lint fiber. The linter fibers are not only short, but also tightly attached to the surface of the seed. Lint fibers can be divided into short fibers (<21mm), medium and long fibers (28-32mm) and long fibers (>35mm). Cotton fiber is protruding and extending from the epidermal cells of the seed. Its growth and development can be artificially divided into four stages: the initiation of fiber (flowering to 5 days after flowering); fiber elong...

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Application Information

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IPC IPC(8): C12N15/29C12Q1/68
Inventor 戎均康何沙娥丁明全李飞飞周伟曹跃芬
Owner ZHEJIANG FORESTRY UNIVERSITY
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