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Technology method for preparing active components of chlorella through scale classification

A technology of active components and process methods, applied in the field of bioengineering, can solve problems such as high difficulty in large-scale preparation, toxic and harmful reagents used, high energy consumption and material consumption, and achieve the effect of realizing value, low equipment requirements, and low energy consumption

Inactive Publication Date: 2014-04-23
东莞市绿安奇生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The chlorella extraction process in the prior art is either limited to the extraction of individual substances, which is likely to cause waste of a large number of other components; or the process is cumbersome, consumes a lot of energy and materials, the use of reagents is toxic and harmful, and large-scale preparation is difficult.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Use fresh chlorella liquid to prepare algae ice raw materials, specifically: centrifuge the cultivated chlorella liquid to obtain concentrated chlorella pulp, and then use plate and frame filter press to remove cells For external water, the filter cloth used for the plate and frame filter press is 250 mesh. After the filter press is completed, the wet algae mud is obtained. It is measured that the water content is about 65±3%; The algae mud is packaged in a stainless steel container and frozen into blocks in a freezer below -10°C for the next step of extraction. The algae mud samples were stored in a refrigerator at -80°C, and after 48 hours of freeze-drying in a vacuum freeze dryer (-40°C), samples were taken to measure the content of protein, fatty acid and chlorophyll a. The content is measured by high-performance gas chromatography, and the content of chlorophyll a is determined by high-performance liquid chromatography; among them, the protein content is 59.42 kg, ...

Embodiment 2

[0036] Use chlorella dry powder to prepare algae ice raw materials, specifically: take 300 kg of chlorella dry powder (water content ~ 5%) and place it in a 3m 3 Add 1,200 kg of water to the batching tank, and stir and infiltrate at a speed of 30 rpm at room temperature for 24 hours. After the chlorella cells completely absorb water and swell, use a plate and frame filter press to remove extracellular water. The plate and frame filter adopts The filter cloth is 250 mesh. After the filter press is completed, the wet algae mud is obtained. After measurement, the water content is 65±3%. The material container is frozen into blocks in a freezer below -10°C for the next step of extraction.

Embodiment 3

[0038] The wall-breaking extraction process is as follows, specifically: using the algae ice of Example 1 or Example 2 as raw material, after the water-containing Chlorella cells are frozen, the intracellular water forms ice crystals, and the cells expand, or even burst the cell wall, so that In broken wall extraction; use 5m 3 The ultrasonic extraction tank (extraction tank with ultrasonic device) is a reaction vessel for wall-broken extraction, add 750 kg of algae ice (about 750L), 1800L of ethyl acetate and 450L of water, at this time the ethyl acetate in the tank: water: algae Ice (v / v / v)=2.4:0.6:1, then ultrasonic treatment, ultrasonic power 2w / cm 2 , the ultrasonic time is 25 minutes, the stirring rate of the extraction tank is 30rpm, and the reaction temperature is controlled below 25°C; when the ultrasonic wave reaches 15 minutes, the algae ice is completely melted, at this time, the sample is diluted, observed under the microscope, and the chlorella cell breakdown is ...

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Abstract

The invention discloses a technology process for preparing active components of chlorella through scale classification. The technology process comprises the steps of preprocessing raw materials, breaking walls for extracting, dissolving lipid, concentrating chlorella oil, drying chlorophyll, extracting growth factors of chlorella and preparing hydrolyzed protein peptide. According to the technology process, products such as coarse chlorella oil, chlorophyll crude product, growth factors of chlorella and hydrolyzed protein peptide are prepared through classification; the technology process is applicable to processing and production of the chlorella higher than pilot plant test level, even kilogram level small batch production and larger scale production. Through the technology process, various functional components of the chlorella are prepared step by step; the obtained components can be further processed, refined and used for developing downstream products so as to realize maximum value of the chlorella; reagents used in the technology process is innocuous and unpoisonous and low in cost; main solvents can be recycled and reused. In addition, the technology process is low in equipment requirement; the production requirement can be met by using normal plant extraction devices; the technology process is short in extraction time, high in efficiency and low in energy consumption.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a process method for large-scale and graded preparation of active components of chlorella, specifically, a method for preparing chlorophyll and algae oil (fatty acid glyceride) by grade using chlorella as raw material , a large-scale production process of chlorella growth factor and chlorella hydrolyzed protein peptide. Background technique [0002] Chlorella is a single-celled green algae, and the common cultured species include Chlorella pyrenoidosa, Chlorella vulgaris, Chlorella protothecoides, etc. Chlorella is a nutritionally comprehensive and balanced human food, which is easily absorbed and utilized by the human body. The protein content is as high as 50% or more, and the amino acid composition is better than that of common protein foods; the chlorophyll content in chlorella can be as high as 2- 4%, which is the food with the highest chlorophyll content; the fatty ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07D487/22C11B1/10C11B1/04C12R1/89
Inventor 俞建中
Owner 东莞市绿安奇生物工程有限公司
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