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A kind of preparation method of bioactive bacteria agent

A live bacterial agent and biological technology, applied in the field of preparation of biological live bacterial agents, can solve the problems of harming the circulatory system, affecting the external image, memory loss, etc., and achieve the effect of simple process steps, deodorization and volume reduction

Active Publication Date: 2016-01-13
毕维
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(3) Harm the circulatory system
(4) Harm the digestive system
(6) Odor can also hinder the good development of interpersonal relationships and affect the external image of individuals, families or a certain place
Long-term exposure to the persistent effects of stench can cause irritability, depression, insomnia, inattention, and memory loss, thereby reducing learning and work efficiency

Method used

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  • A kind of preparation method of bioactive bacteria agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Extract photosynthetic bacteria, yeast, lactic acid bacteria, Bdellovibrio phage, and Bacillus subtilis from various strains in the national database by streaking method.

[0024] 2) Inoculate the yeast into the Erlenmeyer flask for culture, inoculate the Bdellovibrio phage into the Erlenmeyer flask for culture, inoculate the Bacillus subtilis into the Erlenmeyer flask for culture, inoculate the photosynthetic bacteria into the semi-closed glass bottle for culture , inoculating lactic acid bacteria into airtight narrow-necked glass bottles for culture; the culture medium for inoculating yeast into conical flasks can adopt conventional wort solid medium, and inoculating bacteriophage Bdellovibrio into conical flasks for cultivation The culture medium can adopt conventional Gaoshi synthetic No. 1 solid medium, and the culture medium that bacillus subtilis can be inoculated into the Erlenmeyer flask can adopt conventional fish peptone medium, and the photosynthetic bacte...

Embodiment 2

[0028] Similar to Example 1, the difference is that the nutrient solution of the expanded culture is composed of 1 ton of bioactive bacteria:

[0029] K H 2 PO 4 400g, Na 2 HPO 4 300g, MgSO 4 ·7H 2 80g, yeast extract 50g, peptone 150g, MgSO 4 500g, CaCl 2 500g, (NH 4 ) 2 SO 4 500g, MnSO 4 4g, NaAc300g, bamboo liquid 300mL, carbon source 600mL, nitrogen source 500mL, the balance is water.

Embodiment 3

[0031] Similar to Example 1, the difference is that the nutrient solution of the expanded culture is composed of 1 ton of bioactive bacteria:

[0032] K H 2 PO 4 500g, Na 2 HPO 4 600g, MgSO 4 ·7H 2 30g, yeast extract 20g, peptone 50g, MgSO 4 800g, CaCl 2 800g, (NH 4 ) 2 SO 4 700g, MnSO 4 3g, NaAc400g, bamboo liquid 350mL, carbon source 650mL, nitrogen source 700mL, the balance is water.

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Abstract

The invention provides a preparation method for a biological active bacterium agent and relates to biological active bacterium agents. The preparation method comprises the following steps: extracting photosynthetic bacteria, saccharomycetes, lactic acid bacteria, bdellovibrio bacteriovorus and bacillus subtilis from various strains of a national database through a plate streak method; inoculating the saccharomycetes into a triangular bottle to be cultured; inoculating the bdellovibrio bacteriovorus into the triangular bottle to be cultured; inoculating the bacillus subtilis into the triangular bottle to be cultured; inoculating the photosynthetic bacteria into a semi-closed thin-opening glass bottle to be cultured; inoculating the lactic acid bacteria to a closed thin-opening glass bottle to be cultured; and mixing the cultured photosynthetic bacteria, saccharomycetes, lactic acid bacteria, bdellovibrio bacteriovorus and bacillus subtilis and carrying out amplification culture and fermentation to obtain the biological active bacterium agent. According to the preparation method, foul smell can be greatly removed so that liquid-state dirt and organic matters are rapidly metabolized; the volume of solid matters is reduced and polluted matters can be rapidly purified; the foul smell environment of the surrounding can be removed, and industrial sewage and domestic sewage are degraded. Furthermore, process steps are simple and the cost is low; the preparation method is suitable for industrial production.

Description

technical field [0001] The present invention relates to a biological active agent, in particular to a preparation method of a biological active agent. Background technique [0002] With the rapid development of social economy and the continuous acceleration of urbanization, the problem of indoor and outdoor environmental pollution has become increasingly prominent. Stench refers to all substances that stimulate the olfactory organs to cause people's unpleasantness and damage the biological environment. As one of the seven typical public hazards (air pollution, water pollution, stench, soil pollution, noise, vibration, and land subsidence), stench has a wide variety of substances, a wide range of influence, and seriously endangers human health. Its toxicity to the human body is Many aspects: (1) harm the nervous system. Long-term stimulation by one or several low-concentration odorous substances will first cause the loss of the sense of smell, and then lead to a dysfunction...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34B01D53/84A01N63/04A01P1/00A01P3/00C12R1/01A61L101/52
CPCY02A50/20
Inventor 毕景阳
Owner 毕维
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