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Microwave-assisted protein acid hydrolysis method for rapid preparation of small peptide amino acids

A microwave-assisted, protein-acid technology, applied in the field of protein processing, can solve the problems of low yield of highly expressed strains, difficult operation of gene fragments, and difficult monitoring work, and achieve low color value, short hydrolysis time, and high hydrolysis efficiency. Effect

Inactive Publication Date: 2016-01-20
CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

DNA recombination technology is currently limited to the production of macromolecular active polypeptides and proteins. It is difficult to operate small molecule gene fragments, its expression efficiency is low, monitoring work is difficult, it is difficult to screen high-efficiency expression strains and the yield is low. In addition, the production of small The types of peptides are also limited

Method used

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  • Microwave-assisted protein acid hydrolysis method for rapid preparation of small peptide amino acids
  • Microwave-assisted protein acid hydrolysis method for rapid preparation of small peptide amino acids
  • Microwave-assisted protein acid hydrolysis method for rapid preparation of small peptide amino acids

Examples

Experimental program
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Effect test

Embodiment 1

[0029](1) Hydrolysis: Heat the freshly dried, mildew-free, and impurity-free defatted soybean protein powder solution at 90°C for 10 minutes to moderately denature the protein and loosen its dense three-dimensional structure; add mass percentage Hydrochloric acid with a concentration of 20% and a sulfurous acid solution with a concentration of 5.5% by mass are mixed in a volume ratio of 1:1, the mass ratio of the denatured protein material to the mixed acid solution is 1:4, the hydrolysis temperature is 90°C, and the power is 600W of microwave Auxiliary hydrolysis time 4h.

[0030] (2) Filtration: After microwave-assisted acid hydrolysis of protein powder, centrifuge and filter while hot. First use an acid-resistant plate and frame filter press for coarse filtration, pass through double-layer nylon cloth, then pass through a microfiltration compressor for microfiltration, and pass through a 0.5μm microfiltration membrane. Neutralize with KOH solution and make organic fertiliz...

Embodiment 2

[0037] (1) Hydrolysis: Heat the freshly dried, mildew-free, and impurity-free degreased camellia seed protein powder solution at 90°C for 20 minutes to moderately denature the protein and loosen its dense three-dimensional structure; add quality Hydrochloric acid with a percentage concentration of 25% and a sulfurous acid solution with a mass percentage concentration of 6% are mixed in a volume ratio of 1:1, the mass ratio of the denatured protein material to the mixed acid solution is 1:5, the hydrolysis temperature is 100°C, and the power is 300W The microwave-assisted hydrolysis time is 6 hours, and samples are taken every half an hour, and intermediate detection is carried out by TCA method, and records are made;

[0038] (2) Filtration: After microwave-assisted acid hydrolysis of protein powder, centrifuge and filter while hot. First use an acid-resistant plate and frame filter press for coarse filtration, pass through double-layer nylon cloth, then pass through a microfi...

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Abstract

The invention discloses a method for rapidly preparing small peptide amino acids by microwave-assisted protein acid hydrolysis. The method comprises: in the microwave environment, performing hydrolysis on a mixed solution of a denatured protein raw material and hydrochloric acid and sulfurous acid, and performing centrifugation filtering, nanofiltration membrane deacidification and ultrafiltration separation to obtain small peptide amino acids with different molecular weight. The preparation method is mild in reaction conditions, short in production period, low in production cost and high in product recovery rate, realizes "three wastes (waste gas, waste water and industrial residue)" zero discharge, and is applicable to industrial production.

Description

technical field [0001] The invention relates to a method for rapidly preparing small peptide amino acids by microwave-assisted protein acid hydrolysis, which belongs to the technical field of protein processing. Background technique [0002] The protein hydrolyzate is peptone, peptide, etc., and the final product is α-amino acid. Peptides are formed by connecting a certain number of amino acids through peptide bonds. Generally, peptides with 2 to 20 amino acids in peptide bonds are called oligopeptides or small peptides, and peptide chains longer than 20 amino acids are called polypeptides. Modern studies have shown that the main form of protein absorption is not in the form of amino acids but in the form of small peptides. The small peptide transport system has the characteristics of fast speed, low energy consumption and not easy to be saturated. The intensity of amino acids absorbed in the form of peptides is higher than that of free amino acids. The absorption intensity...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/12C07K1/34
Inventor 彭红建阳纯
Owner CENT SOUTH UNIV
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