Ensiling composition containing perennial ryegrass and vicia sativa and application of ensiling composition
A technology of arrowhead pea and composition, which is applied in the field of silage composition, can solve the problems of low utilization rate of arrowhead pea, and achieve the effects of low cost, improved fermentation quality, and easy utilization
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Embodiment 1
[0019] Embodiment 1: screening suitable mixing ratio
[0020] 1 Material method
[0021] 1.1 Test material
[0022] The test materials were planted in the experimental field of Grassland Station, Shigatse City, Tibet Autonomous Region, and mowed on August 24, 2010. The perennial ryegrass (Perennial Ryegrass) was mowed on the stalk after the seeds were harvested, and the arrowhead pea (Vicia sativa L.) was mowed. The time of cutting is the heading period. After harvesting, they were quickly sent back to the laboratory of Shigatse Grassland Station to make silage. The silage cellar uses a laboratory silage tank with a 1L plastic container.
[0023] 1.2 Experimental design
[0024] This experiment is divided into control group and three treatment groups, control group (Control): 100% perennial ryegrass; treatment one (HJ15): 85% perennial ryegrass + 15% arrowhead pea; treatment two (HJ25): 75% Perennial ryegrass + 25% Arrowhead pea; Treatment 3 (HJ35): 65% perennial ryegrass...
Embodiment 2
[0043] Embodiment 2: Screening of suitable biological additives
[0044] 1. Material method
[0045] 1.1 Test material
[0046] 75% perennial ryegrass + 25% arrowhead pea as material.
[0047] The cellulase preparation is cornzyme cellulase (Bao Kangsheng), the main components include cellulose, hemicellulose and glucose oxidative cellulase. It is a special additive for silage developed by Finnish Feed International Co., Ltd., and the dosage refers to the instructions for use. The lactic acid bacteria compound additive is composed of 60% Lactobacillus plantarum freeze-dried powder, 20% Bacillus subtilis freeze-dried powder and 20% glucose by weight. Lactobacillus plantarum and Bacillus subtilis were purchased from Shanghai Fusheng Biotechnology Co., Ltd.
[0048] The preparation method of the lactic acid bacteria compound additive is as follows:
[0049] 1) MRS medium: peptone 10.0g; beef extract 10.0g; yeast extract 5.0g; glucose 20.0g; Tween 1mL; ammonium citrate 2.0g; so...
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