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Poultry enzyme including neutral protease and preparation method thereof

A technology of neutral protease and acid protease, which is applied in the field of enzyme preparation, can solve the problems of unmeasured and complex structure, and achieve the effect of wide applicable pH range and high temperature tolerance

Active Publication Date: 2014-04-09
HUNAN HONGYING BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reason is that the structure of the market is relatively complex, the market is diversified, and it is even impossible to measure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] A poultry enzyme containing neutral protease, consisting of the following enzyme preparations in parts by weight:

[0058] Bacillus subtilis culture 35 parts, acid xylanase 50 parts, acid protease 25 parts, phytase 25 parts, beta-glucanase 17 parts, cellulase 12 parts, amylase 12 parts, pectinase 8 parts, 12 parts of Chinese herbal medicine extract, 12 parts of protective agent, 12 parts of activator.

[0059] Described acid xylanase, acid protease, phytase, β-glucanase, cellulase, amylase, pectinase are all food-grade enzyme preparations;

[0060] The preparation method of described bacillus subtilis culture comprises the steps:

[0061] (1) Inoculate the well-preserved slant strain of Bacillus subtilis 1398-2-12 on the slant medium, culture at 30°C for 24 hours to activate the strain, and activate it twice;

[0062] The slant medium consists of: beef extract 3g, sodium chloride 5g, peptone 10g, glucose 2g, (NH) 2 SO 4 3g,K 2 HPO 4 6g, CaCl 2 1g, 15g agar, 5g Ch...

Embodiment 2

[0096] A poultry enzyme containing neutral protease, consisting of the following enzyme preparations in parts by weight:

[0097] Bacillus subtilis culture 30 parts, acid xylanase 40 parts, acid protease 20 parts, phytase 20 parts, beta-glucanase 15 parts, cellulase 10 parts, amylase 10 parts, pectinase 5 parts, 10 parts of Chinese herbal medicine extract, 10 parts of protective agent, 10 parts of activator.

[0098]Described acid xylanase, acid protease, phytase, β-glucanase, cellulase, amylase, pectinase are all food-grade enzyme preparations;

[0099] The preparation method of described bacillus subtilis culture comprises the steps:

[0100] (1) Activation of strains

[0101] The well-preserved slant strain of Bacillus subtilis 1398-2-12 was inoculated on the slant medium, cultured at 33°C for 30 hours to activate the strain, and activated twice in this way;

[0102] The slant medium consists of: beef extract 6g, sodium chloride 8g, peptone 15g, glucose 4g, (NH) 2 SO 4...

Embodiment 3

[0135] A poultry enzyme containing neutral protease, consisting of the following enzyme preparations in parts by weight:

[0136] Bacillus subtilis culture 40 parts, acid xylanase 60 parts, acid protease 30 parts, phytase 30 parts, beta-glucanase 20 parts, cellulase 15 parts, amylase 15 parts, pectinase 10 parts, 15 parts of Chinese herbal medicine extract, 15 parts of protective agent, 15 parts of activator.

[0137] Described acid xylanase, acid protease, phytase, β-glucanase, cellulase, amylase, pectinase are all food-grade enzyme preparations;

[0138] The preparation method of described bacillus subtilis culture comprises the steps:

[0139] (1) Activation of strains

[0140] The well-preserved slant strain of Bacillus subtilis 1398-2-12 was inoculated on the slant medium, cultured at 36°C for 36 hours to activate the strain, and activated 3 times in this way;

[0141] The slant medium consists of: beef extract 10g, sodium chloride 12g, peptone 20g, glucose 5g, (NH) 2...

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Abstract

The invention discloses a poultry enzyme including neutral protease and a preparation method of the poultry enzyme, and belongs to the technical field of enzyme preparation. The poultry enzyme is formed by bacillus subtilis culture, acid xylanase, acid protease, phytase, beta-dextranase, cellulase, amylase, pectinase, Chinese herb extracts, a protective agent and an activating agent through scientific formulation, wherein the bacillus subtilis culture can be used for improving the immunity of the animal organism, promoting the development of immune organs, promoting the maturity of intestinal track structure and function of the animals, increasing daily gain of the animals and improving the forage conversion rate. The poultry enzyme provided by the invention provides security digestive enzyme for poultries, so that the digestion burden can be effectively reduced, the use rate and growth rate of the raw materials can be improved, and the environment is protected; meanwhile, the suitable amount of an activation agent can sufficiently activate the efficiency of the enzyme in the same conditions, so that the enzyme additive amount is saved. Moreover, the Chinese herb extracts prolong the expiration period of the composite enzyme through the scientific formulation, and also improve the immunity of the fed poultries.

Description

technical field [0001] The invention belongs to the technical field of enzyme preparation, in particular to a poultry enzyme containing neutral protease and a preparation method thereof. Background technique [0002] There are four main reasons for adding enzyme preparations to feed: 1. Degradation of anti-nutritional factors present in animal feed. These substances cannot be degraded by animal endogenous enzymes, thereby interfering with the normal metabolism of animals, resulting in indigestion of animals and decreased production performance. 2. Improve the utilization rate of starch, protein and minerals. These substances are either surrounded by fiber-rich cell walls, or are present in certain structures that cannot be digested by animals (for example, in plant feed materials, a large amount of phosphorus is present in the form of phytate phosphorus.). 3. Degrade some specific chemical bonds in raw materials. These chemical bonds cannot be degraded by the animal's own...

Claims

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Application Information

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IPC IPC(8): A23K1/165A23K1/16C12N1/20C12R1/125
Inventor 李洪兵朱永明易继云刘波樊淳红
Owner HUNAN HONGYING BIOTECHNOLOGY CO LTD
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