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Rabbit serum immunoglobulin liquid-phase suspension chips and preparation and application thereof

An immunoglobulin and suspension chip technology, which is applied in the field of IgG, rabbit serum immunoglobulin IgM, and IgA liquid phase suspension chips, can solve problems such as unavailable, limited detection of rats and mice, and application limitations of liquid phase chip technology.

Inactive Publication Date: 2014-04-02
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Liquid-phase suspension chip technology is a new type of chip technology that can simultaneously perform qualitative and quantitative analysis of multiple indicators. However, as an advanced operating technology platform for bioinformatics, the application of liquid-phase chip technology in the field of basic life science research is still limited.
At present, foreign countries have launched commercialized liquid-phase chip kits that can be applied to experimental animal research, but they are only limited to the detection of rats and mice, and the detection of serum indicators in experimental rabbits cannot be applied.

Method used

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  • Rabbit serum immunoglobulin liquid-phase suspension chips and preparation and application thereof
  • Rabbit serum immunoglobulin liquid-phase suspension chips and preparation and application thereof
  • Rabbit serum immunoglobulin liquid-phase suspension chips and preparation and application thereof

Examples

Experimental program
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Embodiment

[0027] 1: Materials and methods

[0028] 1.1 Experimental animals: 20 JW rabbits, 20 WHBE rabbits, half male and half male, weighing about 2-2.5k, provided by the experimental rabbit production base of Zhejiang University of Traditional Chinese Medicine [SCXK (Zhejiang) 2009-0042].

[0029] 1.2 Sample preparation: Take appropriate amount of blood from the middle ear artery of WHBE rabbits and JW rabbits, put them in 10ml glass tubes, centrifuge at 3000r / m at 4°C for 10min, separate the serum, and store at -20°C.

[0030] 1.3 Experimental reagents: affinity-purified IgG capture antibody, IgM capture antibody, and IgA capture antibody were all purchased from American Bethyl Company, biotin-labeled IgG detection antibody, IgM detection antibody, and IgA detection antibody were all purchased from American Bethyl Company, Streptavidin-PE Antibodies were purchased from Invitrogen Company of the United States, Sulfo-NHS and EDC were purchased from Thermo Company of the United States,...

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PUM

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Abstract

The invention relates to the technical field of biology, and particularly relates to rabbit serum immunoglobulin IgM, IgG and IgA liquid-phase suspension chips and a preparation and application thereof. The rabbit serum immunoglobulin liquid-phase suspension chips comprise 42#, 48# and 57# microspheres which are respectively coupled to IgG, IgM and IgA capture antibodies. In addition, the rabbit IgM, IgG and IgA liquid-phase chips are prepared by the method, the IgM, IgG and IgA contents in WHBE (White Hair and Black Eyes) rabbit and JW rabbit are synchronous detected, and a method for detecting the rabbit serum immunoglobulin IgM, IgG and IgA liquid-phase suspension chips is built. The method for detecting the rabbit serum immunoglobulin IgM, IgG and IgA liquid-phase suspension chips can be applied to research and development of immunology and a biological diagnostic kit, and experimental materials and new resources are provided for development of the bioengineering and pharmaceutical industry, especially research and development and production of biological diagnostic reagents.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a rabbit serum immunoglobulin IgM, IgG and IgA liquid-phase suspension chip and its preparation and application. Background technique [0002] Experimental rabbits are currently one of the most commonly used experimental animals in the field of biomedical product development and medical science research, especially in the preparation of biological antibodies, ophthalmic surgery research, etc., playing an incomparable advantage over rats and mice. The level of serum immunoglobulin Ig is an important indicator to reflect the immune status of the animal body. The change of Ig content is closely related to the disease. Among them, IgG, IgM and IgA are the main effector molecules mediating humoral immunity. [0003] Liquid phase suspension chip technology is a new type of chip technology that can simultaneously perform qualitative and quantitative analysis of multiple indicators. However,...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/531
CPCG01N33/6854G01N33/54313
Inventor 陈民利蔡月琴
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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