Detachable antiskid chromatographic column

Abstract

The invention provides a detachable antiskid chromatographic column, belonging to the field of biological purification instruments. According to the chromatographic column provided by the invention, the top end of the column body is provided with a U-shaped screen, so that a buffer solution, a sample solution, an eluate and other liquids can conveniently flow down along the wall of the column body, thereby avoiding splashing and influencing the chromatographic effect; and the external surface of the column is provided with an antiskid plastic outer sleeve, so that the column body can be held by hands or fixed to a bracket, and the chromatographic column can not be crashed easily, thereby preventing the clip from leaving scratches on the column body made of glass. The column head and column tail are detachable from the column body; and the chromatographic column can be assembled before chromatography, and can be detached after chromatography, so that the column head, column tail and column body can be respectively cleaned, sterilized and the like. The chromatographic column has the advantages of simple structure and favorable protein purification effect, and is convenient to operate.

Description

[0001] technical field [0002] The invention belongs to biological purification equipment, in particular to a detachable anti-slip chromatography column. Background technique [0003] At present, the separation and purification of various compound compositions, natural products, and traditional Chinese medicines are mainly achieved by column chromatography, using a chromatography column, and through a column chromatography process. In traditional column chromatography, the mixed compound is placed on the top of the glass column bed, so that the eluent (mobile phase) composed of the mixed solvent passes through the vertical glass column equipped with an adsorptive medium material (stationary phase) under the action of gravity, Separate and obtain each relatively pure product (pure product), the principle is: due to the different affinity of each component in the mixed compound to the stationary phase and mobile phase in the chromatographic column, the appropriate station...

AI Technical Summary

Problems solved by technology

At present, the common glass chromatography columns have the following problems: the outer surface of the glass chromatography column is relatively smooth, and when the chromatography column is held by hand or fixed with a clip, it is easy to fall, break the chromatography column, and make the its precious stuffing spilled

[0005] In addition, when the current chromatographic column is injected, the sample solution flows directly into the column from the middle of the top of the chromatographic column, which easily causes the filler in the column to splash onto the inner wall of the chromatographic column

Since the packing material used for chromatography is often relatively expensive, the packing material splashed on the inner wall is not fully utilized in the chromatography, which affects the chromatographic effect

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