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Ethyl acetate extract of radix sophorae tonkinensis, as well as preparation method, detection method and application thereof

A technology of ethyl acetate extraction and ethanol reflux extraction, which is applied in the field of ethyl acetate extract of mountain bean root and its preparation, detection and application, to achieve obvious effect and inhibit the activity of butyrylcholinesterase

Active Publication Date: 2014-04-02
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the ethyl acetate extract of the mountain bean root is used for inhibiting butyrylcholinesterase and its detection method, the invention provides a kind of ethyl acetate extract of the mountain bean root and its preparation, detection method and application

Method used

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  • Ethyl acetate extract of radix sophorae tonkinensis, as well as preparation method, detection method and application thereof
  • Ethyl acetate extract of radix sophorae tonkinensis, as well as preparation method, detection method and application thereof
  • Ethyl acetate extract of radix sophorae tonkinensis, as well as preparation method, detection method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Embodiment 1: the ethyl acetate extract of mountain bean root

[0043] Grind bean root into coarse powder, pass through a 35-mesh sieve, weigh 500g of bean root coarse powder, soak in 1500ml of petroleum ether for 24 hours, and then percolate with petroleum ether until the color of the leachate becomes lighter (approx. 2500ml), recover the residue, evaporate the petroleum ether in the residue, and then use 80% ethanol to reflux to extract twice. The amount of ethanol is 2500ml each time, and the extraction time is 2 hours each time. No alcohol smell, then extracted with the same volume of ethyl acetate as the concentrated solution, collected the supernatant, and evaporated to dryness to obtain 1.02g of ethyl acetate extract (yield: 0.204%).

Embodiment 2

[0044] Embodiment 2: the ethyl acetate extract of mountain bean root

[0045]Grind bean root into coarse powder, pass through a 20-mesh sieve, weigh 500g of bean root coarse powder, soak in 2500ml of petroleum ether for 48h, and then infiltrate with petroleum ether until the percolation liquid becomes lighter in color (approximately 3000ml of leachate is collected ), recover the residue, volatilize the petroleum ether in the residue, then use 75% ethanol to reflux to extract twice, each time with 2000ml of ethanol, each extraction time is 2 hours, combine the extracts, then concentrate to no alcohol smell, and then Extract with ethyl acetate equal to the volume of the concentrated solution, collect the supernatant, and evaporate to dryness to obtain 1.7 g of ethyl acetate extract (yield: 0.34%).

Embodiment 3

[0046] Embodiment 3: the ethyl acetate extract of mountain bean root

[0047] Grind bean root into coarse powder, pass through a 25-mesh sieve, weigh 500g of bean root coarse powder, soak in 2000ml of petroleum ether for 24 hours, and then percolate with petroleum ether until the percolation liquid becomes lighter (approximately 5000ml), recover the residue, evaporate the petroleum ether in the residue, and then use 70% ethanol to reflux extract twice, each time with 1500ml ethanol, each extraction time is 2 hours, combine the extracts, and then concentrate until no alcohol smell , and then extracted with the same volume of ethyl acetate as the concentrated solution, collected the supernatant, and evaporated to dryness to obtain 1.36 g of ethyl acetate extract (yield: 2.61%).

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Abstract

The invention relates to an extract of radix sophorae tonkinensis, as well as a preparation method, a detection method and application thereof, and in particular relates to an ethyl acetate extract of radix sophorae tonkinensis, as well as a preparation method, a detection method and application thereof. The ethyl acetate extract is prepared by virtue of the following steps: immersing the radix sophorae tonkinensis with petroleum ether; leaching petroleum ether, recycling residues, and volatizing the petroleum ether in the residues; performing reflux extraction with ethanol, and concentrating the extract until the extract does not have smell of alcohol; extracting the concentrated solution with ethyl acetate of which the volume is the same as that of the concentrated solution; collecting the supernate, and evaporating to obtain the ethyl acetate extract. In vitro tests discover that the ethyl acetate extract of radix sophorae tonkinensis provided by the invention can be used for inhibiting the activity of butyrylcholine esterase, and is remarkable in effect.

Description

[technical field] [0001] The invention relates to an extract of the root of the mountain bean and its preparation, detection method and application, in particular to an ethyl acetate extract of the root of the mountain bean which has the function of inhibiting butyrylcholinesterase and its preparation, detection method and application. [Background technique] [0002] Alzheimer's disease (AD), also known as senile dementia, is a common chronic degenerative neurological disease in the elderly. According to its recognized pathology-cholinergic theory, the use of cholinesterase inhibitors to increase the level of acetylcholine in AD patients is currently the only generally recognized drug treatment. The clinical application of acetylcholinesterase (AchE) inhibitors has relatively large peripheral side effects, and the long-term treatment effect is not ideal. Recent studies have shown that the administration of selective BuChE inhibitors into the cerebral cortex of rats can incr...

Claims

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Application Information

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IPC IPC(8): A61K36/489A61P25/28G01N30/88
Inventor 陈士林黄林芳王冬梅刘艾林徐宏喜杨大坚卞兆祥陈新滋
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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