Inducing medium and method for improving survival rate of butterfly orchid pedicel

Abstract

The invention provides an inducing medium and a method for improving a survival rate of a butterfly orchid pedicel. The inducing medium is prepared by adding agar, white granulated sugar, 6-benzylaminopurine, naphthylacetic acid, carbenicillin, coconut milk and activated carbon in a 1 / 2 MS (Murashige-Skoog) culture medium. The inducing medium comprises the following ingredients at concentration: 7-9 g / L of agar, 15-30 g / L of white granulated sugar, 0-50 mg / L of carbenicillin, 1.5-3 mg / L of 6-benzylaminopurine, 0.2-0.5 mg / L of naphthylacetic acid, 0-100 g / L of coconut milk, and 1-3 g / L of activated carbon. The inducing medium can be used for inducing the butterfly orchid pedicel. The method for improving the survival rate of the butterfly orchid pedicel is low in cost, and low in the sterilizing contamination rate, and can effectively improves the survival rate of the sterilized explant.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture industrialized seedling cultivation, and in particular relates to a method for effectively improving the survival rate and induction effect of the Phalaenopsis pedicels when using the Phalaenopsis pedicels as explants for primary culture. Background technique [0002] Phalaenopsis B1.spp. is a tropical aerial orchid, which has large flowers, long flowering period, gorgeous flower color, rich color, beautiful and unique flower shape, dancing like a butterfly, hence the name. Known as the "Queen of Orchids" among tropical orchids, it is one of the most popular catayas in recent years. [0003] Phalaenopsis is a single-stem aerial orchid, and the plants rarely develop side buds, and the seeds are extremely difficult to germinate. Its conventional propagation is very slow, so tissue culture is often used for rapid propagation. Disinfection of explants is the first step in tissue rapid pr...

AI Technical Summary

Problems solved by technology

At present, in the process of using pedicel axillary buds as explants to induce the cultivation of Phalaenopsis, there are often problems such as incomplete disinfection during the initial induction culture, great damage to the explants during sterilization, and low survival rate of the pedicel.

Carbenicillin is an antibiotic, which is mostly used in medicine at present, mainly for inflammation caused by Pseudomonas aeruginosa, some Proteus, Escherichia coli, etc. It is rarely used in tissue culture applications

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