Lincomycin chemiluminiscent enzyme-linked immunosorbent assay rapid detection kit

Abstract

The invention relates to a detection kit, in particular relates to a lincomycin chemiluminiscent enzyme-linked immunosorbent assay rapid detection kit. The rapid detection kit comprises a kit body, an enzyme plate arranged in the kit body and a reagent in the kit body, wherein all holes of the enzyme plate are coated with envelope antigens, the envelope antigens are prepared from lincomycin metabolites and ovalbumin by coupling; the reagent comprises a lincomycin monoclonal antibody, a horseradish peroxidase labeled goat-anti-mouse antibody, lincomycin-series standard solutions, a condensed phosphate buffer solution, a condensed washing solution and a luminous solution. The detection kit can be used for detecting residual lincomycin and determining the remnant of the lincomycin by adopting the steps of adding samples, washing, adding the horseradish peroxidase labeled goat-anti-mouse antibody, washing, adding the luminous solution, judging the detection results and the like.

Description

technical field [0001] The invention relates to a detection kit, specifically a lincomycin chemiluminescent ELISA rapid detection kit. Background technique [0002] At present, the techniques for detecting lincomycin antibiotic residues in milk mainly include the following methods: [0003] (1) Microbial obstruction detection method, also known as microbiological inhibition test (MIT for short), qualitatively or quantitatively determines the amount of antimicrobial drug residues in the sample according to the inhibitory effect of antibiotics on the physiological functions and reproductive metabolism of specific microorganisms. It is a classic and widely used method for detecting antibiotics in milk. my country's standard for the detection of antibiotic residues in fresh milk (GB4789.27-2003), the antibacterial zone test and turbidity test commonly used abroad in the 1970s and 1980s, and the discoloration detection kits currently on the market are all such. Different types ...

AI Technical Summary

Problems solved by technology

[0005] After the 1990s, the vast majority of physical and chemical detection methods for the determination of lincomycin residues in food mainly rely on liquid chromatography for separation, followed by chromatography / mass spectrometry, gas chromatography, and high-performance thin-layer chromatography. and other detection methods, because of their unique performance, have a little application in the detection of antibiotic residues

However, because antibiotics are haptens, the antigen construction technology is difficult, the immune specificity is strong, and the detection cost is high. At present, IDEXX Laboratory Company, Chahu Sciences and other companies have relatively mature research on the free analysis method, and this technology is still in research and development in China. middle

At present, enzyme-linked immunosorbent assay, radioimmunoluminescence assay, fluorescence immunoassay, chemiluminescence method, etc. are mainly used. Comparing the sensitivity of several marker immunological techniques shows that enzyme immunoassay has a lower sensitivity than luminescence.