Method for nucleic acid sequencing

Abstract

The invention discloses a method for nucleic acid sequencing through adopting nuclease digestion reaction. The method includes the following steps: (1), performing digestion and degradation on a nucleic acid target sequence through an enzyme, so as to generate an enzyme-digested product; (2), detecting the enzyme-digested product, and finally obtaining information of the nucleotide sequence. The method provided by the technical scheme is used for cutting the target sequence and determining an unknown sequence through the exonuclease, and is realized through utilizing the biodegradability of the exonuclease and sequencing the enzyme-digested product generated from degradation, so as to determine the enzyme-digested product.

Description

technical field [0001] The invention relates to a method for gene sequencing, in particular to a method for measuring unknown sequences by cutting target sequences with exonuclease. Background technique [0002] In the 1970s, the British biochemist Sanger and the American biochemist Gilbert established two nucleic acid sequencing technologies based on different principles, the DNA terminal termination method and the chemical degradation method, which created a precedent for the development of modern nucleic acid sequencing technology. Sanger and Gilbert both won the Nobel Prize in Chemistry for this. [0003] The basic principle of the chemical degradation method is that the chemical reagents process the final DNA fragments, resulting in base-specific cleavage, producing a set of reaction mixtures with various lengths of DNA chains, which are separated by gel electrophoresis, and finally sequenced through data analysis . Chemical degradation methods have been stalled becau...

AI Technical Summary

Problems solved by technology

The steps are cumbersome and the consumption of reagents is large

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