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Molecular marker for Solanum melongena anti-greensickness gene and application of molecular marker

A Verticillium wilt resistance and molecular marker technology, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of technical difficulty and high gene homology, and achieve the effect of improving breeding efficiency

Inactive Publication Date: 2014-03-26
刘军
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Problems solved by technology

Transformation of tomato Vel and Ve2 genes into potatoes successfully enhanced its resistance to the highly pathogenic Verticillium black and white, and the CAPS marker developed based on the Ve gene sequence was also successfully applied to the identification of potato resistance to Verticillium wilt. However, the gene homology of eggplant with anti-virus and cultivated eggplant is very high, and it is technically difficult to obtain a significant difference between anti-virus eggplant and non-anti-virus eggplant.

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  • Molecular marker for Solanum melongena anti-greensickness gene and application of molecular marker
  • Molecular marker for Solanum melongena anti-greensickness gene and application of molecular marker
  • Molecular marker for Solanum melongena anti-greensickness gene and application of molecular marker

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Embodiment Construction

[0015] The invention provides a kind of detection breeding material whether contains the allelic PCR marker of Verticillium dahliae resistance gene S1Ve, and specific implementation method is as follows:

[0016] 1) DNA extraction

[0017] Sow the seeds of S.linnaeanum, cultivated eggplant, and the backcross population of S.linnaeanum and Suzhou horn hybrid configuration. When the seedlings grow to 2-3 leaves, take an area of ​​about 1crn 2 DNA was extracted from the delicate leaves of the leaves by the CTAB method, and the DNA concentration was adjusted to about 50ng / ul.

[0018] 2) PCR amplification

[0019] The reaction system includes 2×Taq Mix (Shanghai Pudi Biotechnology Co., Ltd.) 10ul, the upstream primer SL842-R is 5'-GAATCTTCACAAGCTCCAGCTCCAT-3'; the downstream primer SL842-L is 5'-CTTTCCTTCAATCATCTCGT-3' (10pmol / L) 0.5ul, DNA50ng, with ddH 2 O complete the 20ul system. The parameters of the PCR program were as follows: pre-denaturation at 94°C for 3 min, followe...

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Abstract

The invention provides a molecular marker for a Solanum melongena anti-greensickness gene and application of the molecular marker. The molecular marker comprises the following primers: an upstream primer SL842-R being 5'-GAATCTTCACAAGCTCCAGCTCCAT-3', and a downstream primer SL842-L being 5'-CTTTCCTTCAATCATCTCGT-3'. A kit provided by the invention has significant guiding function on the culture of new germplasm and new species of anti-greensickness Solanum melongena, the breeding process is accelerated, the breeding cost is reduced, and relatively high application value is achieved.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and specifically relates to a molecular marker of Verticillium wilt resistance gene S1Ve derived from wild eggplant S. linnaeanum and its application. Background technique [0002] Eggplant (Solanum melongena) is an important worldwide vegetable crop and one of the main vegetable crops in protected cultivation in my country. Verticillium wilt of eggplant is a typical soil-borne disease mainly caused by Verticillium dahliae. Its main symptoms are yellow patch type and reticular patch type on the leaves, resulting in wilting and dwarfing of plants. Wait. In recent years, with the scale-up of protected eggplant production, the development of industrialization and the inevitable continuous cropping, the disease has become an important disease of protected eggplant cultivation, and its occurrence and damage have become more and more serious. Therefore, the demand for eggplant varieties resista...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 刘军
Owner 刘军
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