Method for co-culturing amanita subfrostiana mycelium by using mycorrhizal fungus and saprobic fungus

Abstract

The invention relates to a method for co-culturing an amanita subfrostiana mycelium by using a mycorrhizal fungus and a saprobic fungus, and belongs to the technical field of macro fungus culture. The method is characterized in that the mycorrhizal fungus lactarius piperatus and the artificially cultured saprobic fungus lentinus edodes are added directly in multiplication media, so that the growth rate of the amanita subfrostiana mycelium can be increased by 9-12 times, and the growth is accelerated obviously. According to the method, a tedious and complex process is not required, the reproduction speed of the mycelium can be increased only by adding a mixture of the two fungi into the media, the effect is obvious, the culture is simple and easy to implement, and the production cost is low. Amanita toxins have potential application in the field of development of novel specific medicines such as anti-tumor medicines, anti-microbial and antiviral medicines, sedative or anaesthetic medicines and like, while are difficult to develop and apply due to bottleneck problems that resources are rare and valuable, artificial acclimation is difficult and the like. The method performs innovation research aiming at pure culture restraining problems such as slower growth of the amanita subfrostiana mycelium and the like, and provides a basis for large-scale culture, artificial acclimation and culture in the future and the like of the mycelium.

Description

technical field [0001] The invention relates to a method for jointly cultivating Amanita amanita mycelium by using mycorrhizal fungi and saprophytic fungi, which belongs to the field of biotechnology, and specifically belongs to the category of indoor cultivation of large poisonous fungi. Background technique [0002] Amanita ( Amanita ) belongs to Basidiomycotina, Phytomycetes, Agaricales, Amanitaceae ( Amanitaceae ), is a more special and valuable worldwide widespread genus among poisonous macrofungi, and its species diversity is very rich. Nearly 400 species have been reported in the world, and nearly 100 species (including subspecies, variants and variants) have been recorded in China. According to research, there are still many species in China that have not yet been studied and named. However, with the emergence of the global ecological crisis, many large fungal resources in our country are in danger, in a serious decline and endangered state, and some species are ...

AI Technical Summary

Problems solved by technology

[0004] At present, the main bottlenecks that lead to the difficulty in the development and sustainable utilization of Amanita are: ① Amanita resources are rare, with small populations, few individuals, low yields, and large distributions. Extremely limited, coupled with sensitivity to the habitat, once the habitat is destroyed, it is easy to disappear or become extinct, and it belongs to a special group of endangered organisms, which increases the difficulty of its further research and utilization; Root fungus, which cannot be artificially cultivated at present

Therefore, there is no toxin product that can be developed on a large scale at home and abroad so far. Peptide toxins used as biochemical reagents are still expensive (about 100,000 US dollars per gram more than 10 years ago—Chen Zuohong et al., 1999), which is difficult to meet the needs of scientific research and application required

[0005] The pure culture of Amanita amanita is the premise or key to ensure the sustainable utilization of Amanita amanita resources, but it is still a problem that is difficult to overcome, and there are many blind spots. Difficulty in inducing filaments and very slow mycelial growth are important links or factors that restrict and affect pure culture