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A kind of cultivation method of Cordyceps militaris

A cultivation method and technology for Cordyceps militaris, applied in the field of cultivation of Cordyceps militaris, can solve the problems of inability to invest in industrialized cultivation, unstable cordycepin content, limited yield and the like

Active Publication Date: 2018-05-08
杨洪利 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, how to increase the content of cordycepin in Cordyceps militaris is being explored by many experts at home and abroad. Although the cultivation technology of Cordyceps militaris is mature, the content of cordycepin is all 0.013% to 0.05%, and the highest content is 0.1% to 1.7%. There is a higher breakthrough, even if there is a breakthrough, the content of cordycepin is very unstable and cannot be put into industrialized cultivation. Natural Cordyceps militaris contains 0.2% of cordycepin, but the output is extremely limited
The cultivation method that can stabilize the content of cordycepin at 1.7%-2.5% has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: A method for cultivating Cordyceps militaris, the strain used is the first-generation parent species preserved in the General Microorganism Center of the China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC NO5577.

[0013] Contains the following steps:

[0014] (1) Preparation of solid Cordyceps militaris: A. Enriched solid medium formula: peeled potato 150-200g, sucrose 15-20g, peptone 1-5g, egg 7-10g, agar 18-20g, vitamin B 1 0.2-0.3g, magnesium sulfate 0.2g, water 1000ml; B. Preparation method: Weigh the above formula raw materials in proportion according to the required quantity, peel and cut potatoes into pieces, boil for 25-35min, filter, and add to the supernatant Add vitamin B, sucrose, peptone, magnesium sulfate, egg whole solution and agar, add water to make up to 1L, then sterilize at 121°C for 20-25min; Or on a slant, at 20°C±1°C, incubate in the dark for 7-10 days, and take it out when the mycel...

Embodiment 2

[0020]Example 2: A cultivation method of Cordyceps militaris, the strain used was purchased from the Edible Fungi Research Institute of Ningyang County Haixin Biotechnology Co., Ltd.: the 2nd generation strain of Space Cordyceps militaris No. 1. Its preparation method is the same as that of Example 1. Divide the culture bottles into 3 groups and put them into different layers of a culture room with a temperature of 18-20°C and a humidity of 60-65% for dark culture. A total of 19g of the product, the test results are shown in Table 2:

[0021] group

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Abstract

The invention belongs to the technical field of fungal cultivation, and provides a method for cultivating Cordyceps militaris in order to increase and stabilize the content of cordycepin in Cordyceps militaris at 1.7%-2.5%. Through the preparation of Cordyceps militaris strains, the preparation of liquid Cordyceps militaris strains, and the cultivation of Cordyceps militaris, the Cordyceps militaris is cultivated in wheat, oats, and rice as the main material of the medium, with peptone, silkworm chrysalis powder, sucrose, and magnesium sulfate. , Potassium dihydrogen phosphate as an auxiliary material in the culture medium prepared by making a solution, and cultured under certain culture conditions to obtain fruiting bodies of Cordyceps militaris. The Cordyceps militaris sporocarp cordycepin content that adopts the method for harvesting of the present invention is higher, reaches 1.7%-2.5%, and is stable at about 2%; The 1‑2 generation mother species of any Cordyceps militaris can be put into use no matter whether it is obtained by self-isolation or purchased from a different place, or it is a preserved strain.

Description

technical field [0001] The invention belongs to the technical field of fungal cultivation, and in particular relates to a cultivation method of Cordyceps militaris. Background technique [0002] Cordyceps militaris is the abbreviation of Cordyceps militaris, also called Cordyceps militaris or chrysalis, common name is not old grass, is a medicinal fungus combined with insects and bacteria, modern rare Chinese herbal medicine, Cordyceps militaris belongs to the fungus phylum, Ascomycetes, Hypocreas, Ergotaceae, Cordyceps. It mainly grows in the northern part of our country. Cordyceps militaris is not only rich in protein and amino acid, but also contains more than 30 kinds of trace elements needed by the human body. It is the best nourishing product. [0003] The cordycepin contained in Cordyceps militaris is an important active ingredient, which has a special medical effect on leukemia. The medical community in the world attaches great importance to it. Extracting cordycep...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00C05G1/00
Inventor 周翔杨洪利胡素花杨国华周文贵杨金花
Owner 杨洪利
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