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Seed quantitative trait locus positioning method based on mixed linear model

A mixed linear model, seed number technology, applied in the direction of electrical digital data processing, special data processing applications, instruments, etc., can solve the unreported environmental effects and gene and environment, no epistasis and gene and environment interaction effects Integrate other issues in one model to achieve improved efficiency and accuracy

Active Publication Date: 2014-03-12
ZHEJIANG UNIV
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Problems solved by technology

[0007] However, so far, neither environmental effects nor gene-environment interaction effects have been reported, nor have methods to integrate epistasis and gene-environment interaction effects into one model

Method used

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  • Seed quantitative trait locus positioning method based on mixed linear model
  • Seed quantitative trait locus positioning method based on mixed linear model
  • Seed quantitative trait locus positioning method based on mixed linear model

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Embodiment Construction

[0063] The present invention will be further explained below in conjunction with specific examples.

[0064] The invention divides the seeds into triploid seeds and diploid seeds based on the method for locating the quantitative trait loci of seeds based on the mixed linear model.

[0065] 1. If the trait being analyzed is primarily controlled by maternal and endosperm genetic effects, methods include:

[0066] (1) Establishment of statistical genetic model:

[0067] According to the characteristics of the seeds and the purpose of the experiment, an appropriate statistical genetic model of the seed traits is established. For a mating mapping population derived from two homozygous parents (P1, P2), it is assumed that genetic experiments are carried out in t different environments, and b blocks are set in each environment. The variation of a certain seed trait is regulated by s QTLs (Q1, Q2,..., Qs) and t epistasis, then the observed value of the seed trait phenotype of the i-...

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Abstract

The invention discloses a seed quantitative trait locus positioning method based on a mixed linear model. The method comprises the step of establishing a statistics genetic model, the step of searching the range of a whole genome for all candidate label sections in which an OTL probably exists, the step of regarding the candidate label sections as a concomitant variable, and searching the range of the whole genome for obvious QTLs and a two interaction label section, the step of regarding the QTLs and the two interaction label section as the concomitant variables and searching an obvious interaction label section for obvious loca of a two interaction epistasis, and the step of obtaining coefficients of various effects in the statistics genetic model based on the QTLs and the loca of the two interaction epistasis and calculating the effect of the loca and estimating the heritability of all loca according to the statistics genetic model. The seeds are divided into diploid seeds and triploid seeds according to the traits of the seeds, the epistasis effect and the effect of interaction of genes and the environment are taken into consideration, and the positions of the seed quantitative trait loca and various effects can be fast estimated in an unbiased mode.

Description

technical field [0001] The invention relates to the field of location of quantitative traits of seeds, in particular to a method for location of quantitative traits of seeds based on a mixed linear model. technical background [0002] Crop seeds are a major source of human food, animal feed, and industrial raw materials. The embryo or endosperm is the main component in the seed. The endosperm is the main organ that stores nutrients, such as carbohydrates, proteins and minerals, and it also provides nutrients for the development of the embryo. The endosperm disappears as some species mature, such as Arabidopsis. Regardless of whether the endosperm disappears or not, it will have an impact on the development of the embryo. In addition, the seeds are grown in the mother plant, so the mother plant will also have an impact on the development of the seeds. The study on the location of quantitative trait loci in seeds has become one of the research hotspots in the field of quan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/12
Inventor 徐海明祁婷曹玉杰祝水金楼向阳
Owner ZHEJIANG UNIV
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