Test strip card based on quantum dot labeling

Abstract

The invention relates to the field of in-vitro diagnosis, particularly a test strip card based on quantum dot labeling with tested object standard curve, coefficient parameters and other detection information, which can quickly quantify sample single component or multicomponent concentration in combination with an instrument with signal detection function. The test strip card for detecting samples has is simple and quick, and has the characteristics of high sensitivity, objective result, high use flexibility and the like.

Description

technical field [0001] The invention belongs to the field of in vitro diagnosis, and specifically relates to a quantum dot marker which has detection information such as a standard curve or coefficient parameters of the tested object, and can quickly quantify the concentration of a single component or multiple components of a sample in combination with an instrument with a signal detection function. test strip card. Background technique [0002] Colloidal gold immunochromatography (Gold-Immunochromatography Assay, GICA) has been widely used in various aspects of immunoassays. It uses a microporous membrane as a carrier, and uses the capillary action of the microporous membrane to make it drop on one end of the membrane strip. The liquid sample slowly seeps to the other end of the microporous membrane. If there are specific antigens or antibodies in the liquid, they can be combined with immunogold and then combined with the corresponding antibody or antigen coated on the micr...

AI Technical Summary

Problems solved by technology

The disadvantages are: (1) It can only detect samples qualitatively, but it is difficult to achieve quantitative samples

(2) It is difficult to detect multiple components of the sample at the same time, and the detection efficiency is low

(3) For some samples with extremely low antigen or antibody content, the color of colloidal gold is very light, it is difficult to judge the result with the naked eye, and the detection sensitivity is low

Although the patent can realize simultaneous detection of multiple components, what is claimed is a method, and no claim is made for the rapid immunochromatographic test strip product based on quantum dot labeling

Moreover, this method has deficiencies: (1) To achieve multi-component detection of samples, multiple corresponding detection lines need to be set on the analysis membrane at the same time, that is, each detection line is only used to detect one indicator in the sample, and the detection efficiency is low

(2) Use ultraviolet light to irradiate the test strip detection strip and the quality control strip to observe the presence or strength of the fluorescent line to determine whether the sample contains the target object. In a true sense, this is still a qualitative detection, or at most It can only achieve the determination of the lowest detectable limit of a certain substance, and cannot fundamentally realize the accurate concentration detection of any component in the sample, and the sensitivity is still low

Storage media recently developed in the information field, such as RFID tags (also known as radio frequency identification tags), IC chips, magnetic codes, bar codes, etc., are used for information access and identification and are now widely used in computers, communications, electronics, commerce, and transportation Control management and other fields, especially RFID tags, small in size, large in storage information capacity, identification without manual intervention, is an ideal means of information access identification, but few people use it for biomedical testing at present

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