Multizone multifunctional nerve dual-mode detection microelectrode array chip and preparation method

Abstract

The invention discloses a multizone multifunctional nerve dual-mode detection microelectrode array chip and a preparation method thereof. The chip comprises an insulation substrate, multiple contacts, multiple strip leads and multiple microelectrode arrays. The invention also provides a preparation method of the multizone multifunctional nerve dual-mode detection microelectrode array chip. According to the technical scheme, not only the decorated electrode surface can be employed for culturing nerve cells for synchronously detecting electrophysiological signals and electrochemical signals of neurotransmitters such as acetylcholine and the like of four-group nerve cells under different experiment parameters in real time, but also the four functional zones are employed for decorating different sensitive materials and further for culturing nerve cells for detecting nerve electrophysiological signals in real time and further for comparing the characteristics of different decoration materials. The chip provided by the invention integrates multiple functions, is simple in technological preparation and convenient to use, and is suitable for laboratories to develop research related to nerve cell culture and multi-parameter nerve dual-mode signal detection of nerve cells.

Description

technical field [0001] The invention relates to the technical field of micro-electromechanical processing of biosensors, in particular to a multi-partition multi-functional neural dual-mode (two mode signals of electrophysiological signal and electrochemical signal) detection micro-electrode array chip and a preparation method thereof. Background technique [0002] Micro-Electro-Mechanical Systems (MEMS) processing technology is developed on the basis of micro-electronics and micro-mechanics, and it plays an increasingly important role in various fields. In recent years, microelectrode arrays (microelectrode, MEA) have provided a long-term, multi-site detection method for cells, which solves the problem of detection in vivo or in vitro by using traditional methods such as patch clamp and glass microelectrodes. Only a few channels of data can be obtained during the electrophysiological signal of nerve cells, and the electrode positioning is difficult and the operation is cumb...

AI Technical Summary

Problems solved by technology

In recent years, microelectrode arrays (microelectrode, MEA) have provided a long-term, multi-site detection method for cells, which solves the problem of detection in vivo or in vitro by using traditional methods such as patch clamp and glass microelectrodes. Only a few channels of data can be obtained during the electrophysiological signal of nerve cells, and the problems of difficult electrode positioning and cumbersome operation

[0003] At present, the MEA used for cultivating nerve cells in foreign laboratories can achieve multi-channel, and the radius of the microelectrode can reach the level of 0.5-1.0um, but there are the following problems in the experimental recording process: On the one hand, only a few channels can obtain information. Neuroelectric signals with a high noise ratio, and the function of neurotransmitter detection cannot be integrated; on the other hand, when carrying out electrophysiological and electrochemical experiments on cultured nerve cells, it is impossible to simultaneously detect the influence of different experimental parameters Simultaneous detection of the effects of different modified materials on nerve cells

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