Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Medication selection and curative effect detection reagent kit of glivec assistance treatment of gastrointestinal stromal tumor

A technology for gastrointestinal stromal tumors and detection kits, which is applied in the field of drug selection and curative effect detection kits for Gleevec adjuvant treatment of gastrointestinal stromal tumors, and can solve the problems of poor curative effect and other issues

Inactive Publication Date: 2014-03-12
XIAMEN UNIV
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Imatinib is the first to inhibit the function of BCR-ABL tyrosine kinase, and is used to treat patients with chronic myelogenous leukemia in different stages.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Medication selection and curative effect detection reagent kit of glivec assistance treatment of gastrointestinal stromal tumor
  • Medication selection and curative effect detection reagent kit of glivec assistance treatment of gastrointestinal stromal tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0016] Example Gleevec Adjuvant Treatment of Gastrointestinal Stromal Tumor Medication Selection and Use of Efficacy Detection Kit

[0017] 1 Prepare a kit including the following components: BCR-ABL gene PCR reaction solution (1200 μL) 1 tube, Taq enzyme system (30 μL) 1 tube, control substance (300 μL) 1 tube.

[0018] 2 Collection, storage and transportation of samples:

[0019] 2.1 Type of sample used: fresh blood.

[0020] 2.2 Sample collection, preservation and transportation: RNA extraction is performed immediately after fresh blood is collected with an anticoagulant tube, or white blood cells are extracted and frozen in liquid nitrogen and stored at -80°C. Samples are shipped on dry ice over long distances.

[0021] 3. Nucleic acid extraction:

[0022] It is recommended to use the MagCore Compact Nucleic Acid Extractor and operate according to the instructions of its matching whole blood RNA extraction kit (MRN-01). Finally, an RNA sample with an elution volume of 2...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a medication selection and curative effect detection reagent kit of glivec assistance treatment of a gastrointestinal stromal tumor, and relates to a detection reagent kit for molecular biology and medicine. The kit comprises BCR-ABL (Abelson proto-oncogene-breakpoint cluster region) gene PCR (polymerase chain reaction) liquid, Taq (thermus aquaticus) enzyme, a control, a matched multiplex PCR amplification procedure and a wrappage. The kit helps to select appropriate drugs for the gastrointestinal stromal tumor and evaluates a curative effect of the individual glivec assistance treatment of the gastrointestinal stromal tumor by detecting whether a glivec drug target site BCR-ALB fusion gene exists, and is applicable to multiple fields of selection of clinical assistance medication, prognosis judgment and the like of the gastrointestinal stromal tumor.

Description

technical field [0001] The invention relates to a detection kit for molecular biology and medicine, in particular to a drug selection and curative effect detection kit for Gleevec adjuvant treatment of gastrointestinal stromal tumors. Background technique [0002] Gastrointestinal stromal tumors (the English full name of gastrointestinal stromal tumors, abbreviated as GIST) originate from the mesenchymal tissue of the gastrointestinal tract. It is a rare tumor that occurs in the digestive tract. It was discovered in 1983 first presented in the year. (Mazur MT, Clark HB. Gastric stromal tumors. Reappraisal of histogenesis [J]. Am J Surg Pathol 1983; 7:507-519). GIST accounts for 1% to 3% of gastrointestinal malignant tumors, the estimated annual incidence is about (10 to 20) / 1 million, and 20% to 30% are malignant. However, with the rapid development of molecular biology, the diagnosis and treatment of GIST have also made great progress. In particular, the current targeted...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/106
Inventor 曾骥孟林辉暖吕晓楠刘祝君
Owner XIAMEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com