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Method for obtaining regenerated plants by tissue culture of stems of cryptomeria fortunei

A technology for tissue culture and plant regeneration, applied in the fields of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of danger, insufficient supply of good seedlings, low seed yield, etc., to maintain good traits, promote health and quickly effect of development

Inactive Publication Date: 2014-03-05
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is helpful to solve the problems of the lack of improved cedar clones in my country, the low genetic gain of seeded improved varieties, low seed yield, difficult and dangerous harvesting, and a serious shortage of good seedlings, and to promote the selection and breeding of fine clones. Large-scale breeding is of great significance

Method used

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  • Method for obtaining regenerated plants by tissue culture of stems of cryptomeria fortunei
  • Method for obtaining regenerated plants by tissue culture of stems of cryptomeria fortunei
  • Method for obtaining regenerated plants by tissue culture of stems of cryptomeria fortunei

Examples

Experimental program
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Effect test

Embodiment 1

[0026] (1) Take the stem material, soak it in detergent solution for 30 minutes, gently brush off the surface dust with a brush, and cut it into independent small branches with terminal buds, the length is 5cm, put the branches in a beaker, and rinse with running water 3h, dry the water, then soak in 0.2% carbendazim solution for 33min, wash twice with distilled water, put it in a sterile culture room for aseptic disinfection, and use 75% alcohol under the ultra-clean workbench After disinfection for 15 seconds, soak in 0.1% mercuric chloride for 7 minutes, then wash with sterile water for 5 times, and blot dry with sterile gauze for later use;

[0027] (2) Induction culture, cut off the 2.5cm stem segment, cut off 2 / 3 of the needle length, and insert it into the induction medium (the induction medium is WPM basic medium + 6-BA1.0mg.L -1 +IBA0.03mg.L -1 + 30g.L sucrose -1 +Agar 7g.L -1 ), cultured in a dark incubator for 100 hours, and then placed in a light incubator for c...

Embodiment 2

[0032] (1) Take the stem material, soak it in detergent solution for 25 minutes, gently brush off the surface dust with a brush, and cut it into independent small branches with terminal buds, the length is 5cm, put the branches in a beaker, and rinse with running water 3h, dry the water, soak in 0.2% carbendazim solution for 30min, wash twice with distilled water, put it in a sterile culture room for aseptic disinfection, and use 75% alcohol under the ultra-clean workbench After disinfection for 10 seconds, soak in 0.1% mercuric chloride for 6 minutes, then wash with sterile water for 4 times, and blot dry with sterile gauze for later use;

[0033] (2) Induction culture, cut off 2cm stem segments, cut off 2 / 3 of the needle length, and insert induction medium (induction medium is WPM basic medium + 6-BA0.8mg.L -1 +IBA0.05mg.L -1 + 30g.L sucrose -1 +Agar 7g.L -1 ), cultured in a dark incubator for 72 hours, and then placed in a light incubator, and the buds began to swell and...

Embodiment 3

[0038] (1) Take the stem material, soak it in detergent solution for 25 minutes, gently brush off the surface dust with a brush, and cut it into independent small branches with terminal buds, the length is 5cm, put the branches in a beaker, and rinse with running water 3h, dry the water, soak in 0.2% carbendazim solution for 30min, wash twice with distilled water, put it in a sterile culture room for aseptic disinfection, and use 75% alcohol under the ultra-clean workbench After disinfection for 10 seconds, soak in 0.1% mercuric chloride for 6 minutes, then wash with sterile water for 4 times, and blot dry with sterile gauze for later use;

[0039] (2) Induction culture, cut off 2cm stem segments, cut off 2 / 3 of the needle length, and insert induction medium (induction medium is WPM basic medium + 6-BA0.5mg.L -1 +IBA0mg.L -1 + 30g.L sucrose -1 +Agar 7g.L -1 ), cultured in a dark incubator for 72 hours, and then placed in a light incubator, and the buds began to swell and ge...

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Abstract

The invention relates to the technical field of propagation of plant tissue culture and particularly relates to a method for obtaining regenerated plants by the tissue culture of stems of cryptomeria fortunei. The method comprises the following steps: (1) disinfecting explants; (2) carrying out induction culture; (3) carrying out propagation culture; (4) carrying out rooting and strong seedling culture; and (5) acclimatizing and transplanting. According to the method, the stems of the cryptomeria fortunei are used as the explants to be used for the tissue culture and high-quality stock plants can be selected for carrying out sampling to rapidly obtain a plurality of good groups with the same genetic background; the good properties of the stock plants can be effectively kept by the tissue culture and the production is not limited by seasons; the method is a main form of industrialized seedling production and can be used for culturing a plurality of good germchits in short period. The technology can powerfully promote the breeding, the propagation, and the popularization and the application of clonal improved varieties of the cryptomeria fortunei and can promote the healthy and rapid development of crytomeria fortunei plantation.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture propagation, in particular to a method for obtaining regenerated plants through tissue culture of cedar stem segments. Background technique [0002] Cryptomeria fortunei (Cryptomeria fortunei) is an evergreen tree of the genus Cryptomeria (Cryptomeria D.Don), also known as peacock fir. It is a unique timber tree species in my country. It is mainly distributed in Sichuan, Zhejiang, Jiangxi and other provinces. Cultivated in all areas. Cedar grows rapidly, with straight trunks and strong adaptability. It is an excellent timber and greening tree species. Cedar is one of the most important artificial afforestation tree species in China. It is widely cultivated in southern China and plays an important role in timber supply, ecological construction and urban and rural greening. [0003] Because the vegetative propagation technology of Cedar cedar has not reached a practical level, the seed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 万雪琴张帆蒋时姣刘海鹰钟宇吴富雨黄金亮张双
Owner SICHUAN AGRI UNIV
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