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Fingerprint spectrum detection method and fingerprint spectrum of Yixinshu preparation

A technology of Yixinshu preparations and fingerprints, which is applied in the field of traditional Chinese medicine detection, achieves the effects of high similarity, high technical content, and avoiding large absorption

Active Publication Date: 2014-02-26
GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Now there are many methods for the determination of active ingredients in Schisandra chinensis, such as non-schisandra alcohol A, schisandra alcohol B, etc., and there are many methods for the determination of active ingredients in Salvia miltiorrhiza, such as tanshinone, danshensu, etc., but how to benefit the heart from a more macro perspective However, there is no report on the method of establishing macroscopic fingerprints based on the chemical components in Shu preparations.

Method used

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  • Fingerprint spectrum detection method and fingerprint spectrum of Yixinshu preparation
  • Fingerprint spectrum detection method and fingerprint spectrum of Yixinshu preparation
  • Fingerprint spectrum detection method and fingerprint spectrum of Yixinshu preparation

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Experimental program
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Effect test

Embodiment 1

[0040] Embodiment 1 of the present invention: the fingerprint detection method of Yixinshu capsule, comprises the following steps:

[0041] (1) Preparation of the test solution: Take 2g of the contents of Yixinshu Capsules, weigh them accurately, place in a 25ml volumetric flask, add 20ml of methanol, extract by ultrasonic for 30min, let it cool, add methanol to the scale, shake well, and pass 0.22μm microporous membrane, take the subsequent filtrate as the test solution;

[0042] (2) Preparation of reference substance solution: Take 3 mg of schisandra alcohol A reference substance, accurately weigh it, place it in a 10ml volumetric flask, add methanol to dissolve and set the volume to the mark, and make a solution with a concentration of 0.3mg / mL, pass through 0.22 μm microporous membrane, take the subsequent filtrate as the reference solution;

[0043] (3) Determination: Chromatographic conditions: use octadecylsilane bonded silica gel as filler, acetonitrile as mobile phas...

Embodiment 2

[0046] Embodiment 2: the fingerprint detection method of Yixinshu Granules, comprising the following steps:

[0047] (1) Preparation of the test solution: Take 2g of Yixinshu granule, weigh it accurately, put it in a 25ml volumetric flask, add 25ml of methanol, extract it by ultrasonic for 40min, let it cool, add methanol to the scale, shake well, and pass through 0.22μm The microporous filter membrane, get continued filtrate as need testing solution;

[0048] (2) Preparation of reference substance solution: Take 3 mg of schisandra alcohol A reference substance, accurately weigh it, place it in a 10ml volumetric flask, add methanol to dissolve and set the volume to the mark, and make a solution with a concentration of 0.3mg / mL, pass through 0.22 μm microporous membrane, take the subsequent filtrate as the reference solution;

[0049] (3) Determination: Chromatographic conditions: using octadecylsilane bonded silica gel as filler, acetonitrile as mobile phase A, and 0.005% pho...

Embodiment 3

[0050] Embodiment 3: the fingerprint detection method of Yixinshu tablet, comprises the following steps:

[0051] (1) Preparation of the test solution: Take 2g of Yixinshu Tablets, accurately weighed, put in a 25ml volumetric flask, add 15ml of methanol, extract by ultrasonic for 20min, let it cool, add methanol to the mark, shake well, filter, take Continue filtrate as need testing solution;

[0052] (2) Preparation of reference substance solution: Take 3 mg of Schizandrin A reference substance, accurately weigh it, place it in a 10ml volumetric flask, add methanol to dissolve and set the volume to the mark, make a solution with a concentration of 0.3mg / mL, filter, Get continued filtrate as reference substance solution;

[0053] (3) Determination: Chromatographic conditions: using octadecylsilane bonded silica gel as filler, acetonitrile as mobile phase A, and 0.005% phosphoric acid aqueous solution as mobile phase B for gradient elution, A:B=20%-90 %8: 0%-10%; flow rate 0....

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Abstract

The invention discloses a fingerprint spectrum detection method and a fingerprint spectrum of a Yixinshu preparation. The fingerprint spectrum detection method comprises the following steps: by taking a schizandrin reference substance as reference, measuring the fingerprint spectrum by adopting high performance liquid chromatography (HPLC). The similarity of the HPLC fingerprint spectrum of the Yixinshu preparation established in the method and a reference spectrum is more than 0.9, the quality of the fingerprint spectrum can be effectively characterized, and the product quality is comprehensively monitored. Moreover, a common mode of the HPLC characteristic fingerprint spectrum of the Yixinshu preparation is established, 15 common peaks are calibrated, the established fingerprint spectrum is high in technical content, the simplicity and one-sidedness of the quality control of the Yixinshu preparation are avoided, and the possibility that the quality of the artificially handled product reaches the standard is reduced.

Description

technical field [0001] The invention relates to a method for detecting fingerprints of Yixinshu preparations and fingerprints thereof, belonging to the technical field of traditional Chinese medicine detection. Background technique [0002] Cardiovascular and cerebrovascular diseases are common diseases that seriously endanger human beings. In recent years, due to social development, changes in work, life, diet and environment, cardiovascular and cerebrovascular diseases are on the rise. For the treatment of cardiovascular diseases, although the single-target effect of traditional Chinese medicine is lower than that of western medicine, its multi-channel, multi-target, dynamic overall treatment, and less toxic and side effects are far beyond the reach of western medicine. Chinese patent medicine with definite curative effect The comprehensive treatment effect of traditional Chinese medicine is more than that of western medicine. Yixinshu preparations are mostly used to tre...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 张观福
Owner GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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