Preparation method of composite biological organic fertilizer granules
A bio-organic fertilizer and granule technology, which is applied in the treatment of bio-organic parts, organic fertilizers, fertilizer mixtures, etc., can solve problems such as unacceptability of enterprises, and achieve full complementarity of functions, significant inhibitory effect, and the effect of reducing pesticide residues.
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Embodiment 1
[0046] A, prepare the bacterial liquid of each bacterial species separately
[0047] (1) Preparation of Bacillus subtilis bacterial liquid:
[0048] Under sterile conditions, Bacillus subtilis CICC 23659 was inserted into the culture medium to carry out liquid fermentation production. Bean cake flour 7.5%, dipotassium hydrogen phosphate 0.2%, ammonium sulfate 0.1%, magnesium sulfate 0.05%, ferric chloride 0.005%, calcium carbonate 0.05%, yeast powder 0.01%, boric acid 0.005%, pH adjusted to 7.2~7.4 , sterilize at about 120°C for 20 min;
[0049] During the fermentation process, the culture temperature range is 28~40℃, and sterile air (dissolved oxygen) is introduced and stirred. The ventilation volume of the sterile air is 0.5~1.0L∕L·min, the stirring speed is 150~200rpm, and the tank pressure is 0.5kg∕cm 2 , oxygen-consuming culture for 48 hours, the number of Bacillus subtilis subsp. Stella in the obtained bacterial liquid is ≥ 2.0 × 10 9 cfu / mL;
[0050] (2) Preparation...
Embodiment 2
[0078] Prepare mixed bacterial liquid in step B, and each bacterial liquid is mixed according to the following proportions:
[0079] Bacillus subtilis 53 parts,
[0080] 12 actinomycetes
[0081] 9 copies of photosynthetic bacteria,
[0082] Paecilomyces lilacinus 8 parts,
[0083] 4 parts of yeast;
[0084] 3 copies of bifidobacteria;
[0085] The rest are the same as in Example 1
Embodiment 3
[0087] Prepare mixed bacterial liquid in step B, and each bacterial liquid is mixed according to the following proportions:
[0088] Bacillus subtilis 55 parts,
[0089] 10 actinomycetes
[0090] 10 copies of photosynthetic bacteria,
[0091] Paecilomyces lilacinus 10 parts,
[0092] 5 parts of yeast;
[0093] 2 copies of bifidobacteria;
[0094] The rest are the same as in Example 1
[0095] The multi-strain microorganism compound flushing fertilization prepared in Examples 1-3 of the present invention was used as flushing fertilization at 10 kg / mu. After the crops are planted, once every 10-20 days, use 2-3 times in total. The application effects are as follows: the leaves are thickened, the flower retention rate is increased by 50%, the fruit is set 7-10 days earlier, the yield is increased by 15-20%, and the incidence of cucumber fusarium wilt is less than 10%.
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