Method for promoting Codonopsis pilosula seed germination by utilizing bacteriabeneficial beneficial bacteria
A technology of seeds and Codonopsis pilosula, applied in the fields of microbiology and plant physiology, can solve problems such as scattered resources, limited quantity of commodities, and contradiction between supply and demand in Codonopsis pilosula market, and achieve simple treatment methods, strong non-pathogenicity and stress resistance, and improved Effects on Vigor and Emergence
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Embodiment 1
[0016] Embodiment 1: a kind of method that rhizosphere beneficial bacteria promotes Codonopsis pilosula seed germination, its main feature is that the steps are:
[0017] (1) Disinfection treatment of Codonopsis pilosula seeds: Soak Codonopsis pilosula seeds with full grains, no defects, and uniformity in 75% alcohol for 8 seconds, then soak in 5% sodium hypochlorite for 10 minutes, rinse with sterilized distilled water for 7-8 times, and store in a refrigerator at 4°C overnight;
[0018] (2) Preparation of Bacillus subtilis GB03 bacterial liquid:
[0019] Prepare LB liquid medium first: prepare according to the ratio of sodium chloride 5g / L, tryptone 10g / L and yeast powder 5g / L, adjust the pH to 6.8 with a standard pH meter (PB-10, Beijing Sartorius), Use a vertical pressure steam sterilizer (LDZX-50KBS, Shanghai Shenan) to sterilize at a high temperature of 118-125 °C and wait for cooling. Add 50 μL of GB03 bacterial solution to every 100 mL of LB liquid medium, and at a sp...
Embodiment 2
[0021] Embodiment 2: a kind of method that rhizosphere beneficial bacteria promotes Codonopsis pilosula seed germination, its main feature is that the steps are:
[0022] (1) Disinfection treatment of Codonopsis pilosula seeds: Soak Codonopsis pilosula seeds with full grains, no defects, and uniformity in 75% alcohol for 9 seconds, then soak in 5% sodium hypochlorite for 11 minutes, rinse with sterilized distilled water for 7-8 times, and store in a refrigerator at 2°C overnight;
[0023] (2) Preparation of Bacillus subtilis GB03 bacterial liquid:
[0024] First prepare lysate broth LB liquid medium: prepare according to the ratio of sodium chloride, tryptone and yeast powder at 1:2:1, the solvent is distilled water; adjust the pH to 7.2, sterilize at 115-118°C at high temperature, and wait for cooling After the temperature is 20°C, add 80 μL of Bacillus subtilis GB03 bacterial liquid to every 100 mL of LB liquid medium, and measure the concentration of the bacterial liquid a...
Embodiment 3
[0026] Embodiment 3: a kind of method that rhizosphere beneficial bacteria promotes Codonopsis pilosula seed germination, its main feature is that the steps are:
[0027] (1) Disinfection treatment of Codonopsis pilosula seeds: Soak Codonopsis pilosula seeds with full grains, no defects, and uniformity in 75% alcohol for 10 seconds, then soak in 5% sodium hypochlorite for 12 minutes, rinse with sterilized distilled water for 7-8 times, and store in a refrigerator at 4°C overnight;
[0028] (2) Preparation of Bacillus subtilis GB03 bacterial liquid:
[0029] First prepare lysate broth LB liquid medium: prepare according to the ratio of sodium chloride, tryptone and yeast powder at 1:2:1, the solvent is distilled water; adjust the pH to 7.2, sterilize at 125-132°C at high temperature, and wait for cooling After the temperature is 35°C, add 100 μL of Bacillus subtilis GB03 bacterial liquid to every 100 mL of LB liquid medium, and measure the concentration of the bacterial liquid...
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