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Active biological matrix product specially used for culture of cucumber seedlings

A technology of biological substrate and seedling-raising substrate, which is applied in the field of agricultural microorganisms, can solve the problems of slow development of seedling-raising biological substrates, and achieve the effects of enhancing the prevention and control of cucumber soil-borne diseases, promoting growth, and promoting growth

Active Publication Date: 2014-01-29
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to develop a special active biological matrix product for cucumber seedlings in view of the slow development of domestic seedling biological matrix

Method used

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  • Active biological matrix product specially used for culture of cucumber seedlings
  • Active biological matrix product specially used for culture of cucumber seedlings
  • Active biological matrix product specially used for culture of cucumber seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, the production of functional bacteria liquid

[0023] The strain SQR9 (CGMCC NO.5808) was inoculated into the PDA culture medium for liquid fermentation production. The conditions for fermentation production were: natural pH, culture temperature 30°C, stirring speed 180 rpm, spores formed in the middle and late stages of fermentation, and the fermentation broth The amount of bacteria or spores in the medium is ≥1×10 9 pieces / ml. The preparation method of the PDA culture medium used is as follows, taking the preparation of 1L medium as an example: Peel 200g of potatoes, cut into small pieces, put them in water and boil, boil for 30min, add 20g of ordinary sucrose to the filtrate after filtration, and dilute to 1000ml, adjust the pH value to 7.0, and sterilize at 121°C for 20min.

Embodiment 2

[0024] Embodiment 2, research and development of functional biomatrix

[0025] 2.1 Seedling raising effect of different inoculated amount of biological matrix

[0026] Research and development of the substrate: the bacterial strain SQR9 (prepared in Example 1) was inoculated into common In the seedling culture medium, mix well, after counting, they contain 1.88×10 7 CFU / g, 8.35×10 7 CFU / g dry weight of functional bacteria. The bacterial strain SQR9 in the matrix is ​​counted by selective medium, and the formula of selective medium is: peptone 10g, yeast powder 5g, NaCl 10g, agar 2.5%, deionized water 1000ml, 1% polymyxin 2ml / L, 1% actin Cytoxanone 4ml / L. The number of colonies of functional bacteria SQR9 (CGMCC NO.5808) is calculated by dry weight per gram of substrate.

[0027] The treatment of seedling raising test is as follows: 1) CK, common seedling raising medium; 2) Seedling raising biological matrix (2%SQR9) added with 2% strain SQR9 bacterial solution; 3) Seedlin...

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Abstract

The invention discloses an active biological matrix product specially used for culture of cucumber seedlings, which is prepared by adding functional bacterial Bacillus amyloliquefaciens SQR 9 in a common seedling matrix, wherein the preservation number of the functional bacterial Bacillus amyloliquefaciens SQR 9 is CGMCC NO. 5808. In the active biological matrix product, cucumber rhizosphere functional bacterial is specially used for cucumber, and is kept alive and added to the matrix to obtain the active biological matrix product specially used for culture of cucumber seedlings. By using the product, the growth promoting effect in the seedling stage of cucumber is good, and high-quality seedlings with a large number of active functional microbes in the rhizosphere can be bred. Meanwhile, a large number of beneficial microbes can be colonized in the rhizosphere so that the growth of the seedlings in the later cultivation process can be promoted, the yield of the crop in field planting can be improved, and the efficacy of preventing cucumber soil-borne disease for the seedlings can be enhanced.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms and relates to a special active biological substrate product for cucumber seedling cultivation. Background technique [0002] With the development of facility agriculture at home and abroad, more and more attention has been paid to industrialized seedling cultivation. According to incomplete statistics, the area of ​​crops that need to be raised in my country is very large, among which the planting area of ​​rice exceeds 300 million mu / year, and the area of ​​economic crops exceeds 500 million mu / year. At present, domestically, it is generally made of materials rich in organic matter (such as peat, decomposed plant residues, etc.), and then mixed with an appropriate proportion of light inorganic materials, such as vermiculite, expanded perlite, etc. Since the source of materials is limited by region and cost, and peat is a non-renewable resource, it limits the sustainable development of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
Inventor 沈其荣张苗李荣钟茜孙逸飞沈标
Owner NANJING AGRICULTURAL UNIVERSITY
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