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Production method of Trichoderma harzianum chlamydospore

A spore and ion solution technology, applied in the field of microbial fermentation, can solve the problems of short shelf life of conidia and low stress resistance of conidia, and achieve the effect of high fermentation unit

Active Publication Date: 2014-01-22
湖南慧谷农业生态研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The shelf life of conidia is relatively short, generally only about 2 to 3 months, shorter in summer, and the stress resistance of conidia is low, which is not conducive to the application of Trichoderma harzianum in agriculture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 solid fermentation produces Trichoderma harzianum chlamydospore

[0020] Include the following steps:

[0021] 1. Method for making seeds required for liquid fermentation

[0022] Trichoderma harzianum strains purchased from the China Agricultural Microorganism Culture Collection Management Center (ACCC) with a preservation number of 31707 were activated on the slope of a PDA test tube, and then transferred to a PDA eggplant-shaped bottle for cultivation at a temperature of 28°C. The culture time is 4 days.

[0023] The liquid activation medium for Trichoderma harzianum slant seeds was prepared, and the medium components were calculated by mass fraction: ammonium sulfate content was 1.5%, glucose content was 1.2%, Tween 80 content was 0.2%, and the balance was water. Prepare medium 250mL according to the above ratio, sterilize at 121°C for 15 minutes, scrape off the slant bacterial lawn of the cultured eggplant bottle in the ultra-clean workbench after co...

Embodiment 2

[0033] Embodiment 2 solid fermentation produces Trichoderma harzianum chlamydospore

[0034] Include the following steps:

[0035] 1. Method for making seeds required for liquid fermentation

[0036]Trichoderma harzianum strains purchased from the China Agricultural Microorganism Culture Collection Management Center (ACCC) with a preservation number of 31707 were activated on the slope of a PDA test tube, and then transferred to a PDA eggplant-shaped bottle for cultivation at a temperature of 28°C. The culture time is 4 days.

[0037] The liquid activation medium for Trichoderma harzianum slant seeds was prepared, and the medium components were calculated by mass fraction: ammonium sulfate content was 1.5%, glucose content was 1.2%, Tween 80 content was 0.2%, and the balance was water. Prepare medium 250mL according to the above ratio, sterilize at 121°C for 15 minutes, scrape off the slant bacterial lawn of the cultured eggplant bottle in the ultra-clean workbench after coo...

Embodiment 3

[0047] Embodiment 3 solid fermentation produces Trichoderma harzianum chlamydospore

[0048] Include the following steps:

[0049] 1. Method for making seeds required for liquid fermentation

[0050] Trichoderma harzianum strains purchased from the China Agricultural Microorganism Culture Collection Management Center (ACCC) with a preservation number of 31707 were activated on the slope of a PDA test tube, and then transferred to a PDA eggplant-shaped bottle for cultivation at a temperature of 28°C. The culture time is 4 days.

[0051] The liquid activation medium for Trichoderma harzianum slant seeds was prepared, and the medium components were calculated by mass fraction: ammonium sulfate content was 1.5%, glucose content was 1.2%, Tween 80 content was 0.2%, and the balance was water. Prepare medium 250mL according to the above ratio, sterilize at 121°C for 15 minutes, scrape off the slant bacterial lawn of the cultured eggplant bottle in the ultra-clean workbench after co...

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Abstract

The invention provides a high-yielding method of Trichoderma harzianum chlamydospore. Trichoderma harzianum is subjected to culture activation, first-level liquid seed solution preparing and second-level liquid seed solution preparing, and then a solid fermentation method is used for fermentation to generate the chlamydospore. A solid fermentation medium for solid fermentation comprises maize straw powder, bean pulp powder and inorganic salt. According to the production method of the Trichoderma harzianum chlamydospore, a large quantity of chlamydospore can be generated, the maximum matrix chlamydospore quantity can reach 1.81 * 109 cfu / g, the fermentation unit is high, waste materials cannot be generated during a production process, environment is protected, and pollution is avoided. The spore-matrix mixture obtained through fermentation can be directly used for crop biocontrol after being dried.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a production method of Trichoderma harzianum chlamydospores. Background technique [0002] Trichoderma (Trichoderma spp.) belongs to the phylum Fungi, Subphylum Deuteromycota, Hyphospora, Synchospora, and Myxospora. It is a class of ubiquitous fungi, commonly found in soil, and is an important community of soil microorganisms. . Trichoderma fungi are widely distributed in nature, and widely exist in decayed wood, seeds, plant residues, and air, and can be isolated from them. [0003] Trichoderma has important economic significance and is widely used in various aspects of agriculture, such as preventing and controlling plant diseases, promoting plant growth, bioremediating soil and environment, and applying to bio-organic fertilizers. In recent years, the research and application of Trichoderma have developed rapidly. Combining the research methods of genetics, molecular b...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12R1/885
Inventor 丰来罗志威郭帅吕黎粟静严征
Owner 湖南慧谷农业生态研究院有限公司
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