Culture method for increasing biomass and grease yield of microalgae

A cultivation method and biomass technology are applied in the cultivation field of improving the yield of microalgae biomass and oil, which can solve the problems of increasing the cost of high-concentration glucose, and achieve the effects of promoting the yield of biomass and reducing the cost.

Active Publication Date: 2014-01-01
博露(厦门)生物股份有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

However, this method is only suitable for microalgae that can grow concurrently, and the addition of high concentrations of glucose increases the cost

Method used

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  • Culture method for increasing biomass and grease yield of microalgae
  • Culture method for increasing biomass and grease yield of microalgae
  • Culture method for increasing biomass and grease yield of microalgae

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Embodiment 1

[0011] Example 1. The specific steps of the cultivation method for improving the yield of N.salina biomass and oil in this example are as follows (see the process flow chart figure 1 ):

[0012] 1. Eutrophic culture of microalgae: Inoculate Pseudochlorococcum N.salina into a normal nutrient-rich f / 2 medium, with an initial pH of 8, a temperature of 24±2°C, and an illumination of 79.29 μmol m -2 s -1 , Ventilation training to logarithmic growth phase. Nutrient-rich f / 2 medium, each liter contains: 33.6g artificial sea salt, 0.075g NaNO 3 , 0.00565g NaH 2 PO 4 ·2H 2 O, 1ml trace elements (T) and 1ml vitamins. Among them, each liter of trace element T contains: 4.16g Na 2 EDTA, 3.15g FeCl 3 ·6H 2 O, 0.18g MnCl 2 ·4H 2 O, 10mg CoCl 2 ·6H 2 O, 10mg CuSO 4 ·5H 2 O, 22mg ZnSO 4 ·7H 2 O, 6mg Na 2 MoO 4 ·2H 2 O. Each liter of vitamins contains: 100mg vitamin B1, 0.5mg vitamin B12 and 0.5mg vitamin H.

[0013] 2. Collection and concentration of microalgae: After the microchlorococcus N. sal...

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Abstract

The invention discloses a culture method for increasing the biomass and grease yield of microalgae in the technical field of algal biological culture. The culture method comprises the following steps: culturing the microalgae in a nutrition-rich culture medium until a logarithmic phase with a nutrition-rich and nutrition-poor two-stage culture method for Nannochloropsis salina at first, then concentrating and collecting the microalgae, and inoculating the microalgae to a phosphorus-free or microelement-free nutrition-poor medium with the microalgae for continuous culture. According to the method, additional nutrition does not need to be added, so that the cost is reduced, the biomass yield, the cell grease content and the final grease yield of the microalgae are greatly increased, and the shortcoming that only the cell grease content is increased but the final grease yield is still very low in a traditional nitrogen-free culture process is overcome; in the absence of phosphorus, the grease content of the microalgae reaches 46% and the biomass and grease yields are increased by 30% and 117% respectively; and in the absence of microelements, the grease content of the microalgae reaches 40% and the biomass and grease yields are increased by 65% and 133% respectively.

Description

Technical field [0001] The invention relates to a cultivation technique of algae organisms, in particular to a cultivation method for improving the production of microalgae biomass and oil. Background technique [0002] The continuous consumption of traditional fossil fuels and the continuous growth of human demand for energy have led to an increasingly serious energy crisis. Studies have shown that starting from 2005, the world's coal, oil, and natural gas will be exhausted in 107, 35, and 37 years. Seeking an environmentally friendly and renewable energy source has become a major problem that needs to be solved urgently by all countries in the world. The use of microalgae to produce biodiesel provides a solution with great potential. Compared with other organisms, microalgae has attracted more and more attention because of its fast growth, small footprint, simple cultivation method, and some of the algae contain high oil content. The use of microalgae to produce biodiesel is...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 陈意民
Owner 博露(厦门)生物股份有限公司
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