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Epidermal growth factor complex liposome and its preparation method and application

A technology of epidermal growth factor and compound lipid, which is applied in the direction of liposome delivery, skin care preparations, chemical instruments and methods, etc., can solve the problem that the skin care and skin health effect of epidermal growth factor cannot be fully exerted, and active substances are susceptible to external conditions. Affect, unable to prevent skin aging and other problems, achieve the effect of reducing pigmentation, muscle fibers arranged neatly and tightly, and reducing scars

Active Publication Date: 2016-01-20
GUANGZHOU GIALEN COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, traditional cosmetics only supplement the external nutrition of the skin and cover the aging skin, and cannot prevent the aging of the skin
Moreover, the actual effect of the nutrients and active cosmetics produced by the general method is not large, because the nutrients and actives are not easy to penetrate into the deep layer of the skin. Normal skin can only absorb some small molecules and fat-soluble substances, and cannot absorb macromolecular proteins. It is more difficult to enter the cell, and the active substance is often inactivated when it is used because it is easily affected by external conditions
As a polypeptide and protein, epidermal growth factor (EGF) is sensitive to enzymes, light, pH, etc., and has the characteristics of poor stability, easy inactivation, and low bioavailability, which greatly limits its application. In order to solve the above problems, Many cosmetics simply encapsulate epidermal growth factor in liposomes to form a single epidermal growth factor liposome for use. Although the effects of enzymes and pH values ​​are isolated and the activity of epidermal growth factor is protected to a certain extent, the use of The rate is still unsatisfactory, and the skin care and skin health effects of epidermal growth factor cannot be fully exerted

Method used

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  • Epidermal growth factor complex liposome and its preparation method and application
  • Epidermal growth factor complex liposome and its preparation method and application
  • Epidermal growth factor complex liposome and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0031] (1) Dissolve 40mg of hydrogenated lecithin, 10mg of cholesterol, 5mg of vitamin E, 5mg of seabuckthorn oil and 5mg of magnolol in 20ml of absolute ethanol. The organic solvent is removed by rotary evaporation, and a uniform lipid film is formed on the bottom of the spinner bottle;

[0032](2) Dissolve 0.1 mg of epidermal growth factor, 10 mg of glycerol and 5 mg of yeast extract in 10 ml of phosphate buffer (pH 6.8), shake well and add to a spinner bottle to rotate the hydrated phospholipid film, remove it for ultrasonication in an ice-water bath treatment and ultrasonication for 30 minutes to fully hydrate the lipid film to obtain epidermal growth factor complex liposome I.

Embodiment 2

[0034] (1) Dissolve 50mg of hydrogenated lecithin, 15mg of cholesterol, 10mg of vitamin E, 10mg of seabuckthorn oil and 15mg of magnolol in 20ml of absolute ethanol. The organic solvent is removed by rotary evaporation, and a uniform lipid film is formed on the bottom of the spinner bottle;

[0035] (2) Dissolve 0.5mg of epidermal growth factor, 15mg of glycerol and 10mg of yeast extract in 10ml of phosphate buffer (pH 6.8), shake well and add to a spinner bottle to rotate the hydrated phospholipid film, remove it for ultrasonication in an ice-water bath treatment and ultrasonication for 40 minutes to fully hydrate the lipid film to obtain epidermal growth factor complex liposome II.

Embodiment 3

[0037] (1) Dissolve 80mg of hydrogenated lecithin, 20mg of cholesterol, 15mg of vitamin E, 15mg of seabuckthorn oil and 15mg of magnolol in 20ml of absolute ethanol. The organic solvent is removed by rotary evaporation, and a uniform lipid film is formed on the bottom of the spinner bottle;

[0038] (2) Dissolve 1 mg of epidermal growth factor, 20 mg of glycerol and 15 mg of yeast extract in 10 ml of phosphate buffer (pH 6.8), shake well and add to a spinner bottle to rotate the hydrated phospholipid film, remove it for ultrasonic treatment in an ice-water bath , sonicate for 50 minutes to fully hydrate the lipid film to obtain epidermal growth factor complex liposome III.

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Abstract

The invention belongs to the field of traditional Chinese medicine, and discloses an epidermal growth factor complex lipidosome, and a preparation method and an application of the lipidosome. The epidermal growth factor complex lipidosome is prepared by the following raw materials in parts by mass: 0.1-5 parts of epidermal growth factor, 5-30 parts of yeast extract, 5-30 parts of magnolol, 5-30 parts of sea buckthron oil, 40-200 parts of hydrogenated lecithin, 10-50 parts of cholesterol, 10-50 parts of glycerol and 5-30 parts of vitamin E. The lipidosome is simple in technology, safe, nontoxic, mild in property and stable in effect, and has small skin irritation.

Description

technical field [0001] The invention belongs to the field of cosmetics, and in particular relates to an epidermal growth factor complex liposome and a preparation method and application thereof. Background technique [0002] At present, traditional cosmetics only supplement the external nutrition of the skin and cover the aging skin, and cannot prevent the aging of the skin. Moreover, the actual effect of the nutrients and active cosmetics produced by the general method is not large, because the nutrients and actives are not easy to penetrate into the deep layer of the skin. Normal skin can only absorb some small molecules and fat-soluble substances, and cannot absorb macromolecular proteins. It is more difficult to enter the cells, and the active substances are often inactivated when they are used because they are easily affected by external conditions. As a polypeptide and protein, epidermal growth factor (EGF) is sensitive to enzymes, light, pH, etc., and has the charact...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A61K8/99A61K8/97A61K8/64A61K8/14A61Q19/00A61Q19/02A61Q19/08C11D9/60C11D9/50C11D9/38C11D9/40
Inventor 候爱霞张彦红
Owner GUANGZHOU GIALEN COSMETICS
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