Epidermal growth factor complex liposome and its preparation method and application
A technology of epidermal growth factor and compound lipid, which is applied in the direction of liposome delivery, skin care preparations, chemical instruments and methods, etc., can solve the problem that the skin care and skin health effect of epidermal growth factor cannot be fully exerted, and active substances are susceptible to external conditions. Affect, unable to prevent skin aging and other problems, achieve the effect of reducing pigmentation, muscle fibers arranged neatly and tightly, and reducing scars
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Embodiment 1
[0031] (1) Dissolve 40mg of hydrogenated lecithin, 10mg of cholesterol, 5mg of vitamin E, 5mg of seabuckthorn oil and 5mg of magnolol in 20ml of absolute ethanol. The organic solvent is removed by rotary evaporation, and a uniform lipid film is formed on the bottom of the spinner bottle;
[0032](2) Dissolve 0.1 mg of epidermal growth factor, 10 mg of glycerol and 5 mg of yeast extract in 10 ml of phosphate buffer (pH 6.8), shake well and add to a spinner bottle to rotate the hydrated phospholipid film, remove it for ultrasonication in an ice-water bath treatment and ultrasonication for 30 minutes to fully hydrate the lipid film to obtain epidermal growth factor complex liposome I.
Embodiment 2
[0034] (1) Dissolve 50mg of hydrogenated lecithin, 15mg of cholesterol, 10mg of vitamin E, 10mg of seabuckthorn oil and 15mg of magnolol in 20ml of absolute ethanol. The organic solvent is removed by rotary evaporation, and a uniform lipid film is formed on the bottom of the spinner bottle;
[0035] (2) Dissolve 0.5mg of epidermal growth factor, 15mg of glycerol and 10mg of yeast extract in 10ml of phosphate buffer (pH 6.8), shake well and add to a spinner bottle to rotate the hydrated phospholipid film, remove it for ultrasonication in an ice-water bath treatment and ultrasonication for 40 minutes to fully hydrate the lipid film to obtain epidermal growth factor complex liposome II.
Embodiment 3
[0037] (1) Dissolve 80mg of hydrogenated lecithin, 20mg of cholesterol, 15mg of vitamin E, 15mg of seabuckthorn oil and 15mg of magnolol in 20ml of absolute ethanol. The organic solvent is removed by rotary evaporation, and a uniform lipid film is formed on the bottom of the spinner bottle;
[0038] (2) Dissolve 1 mg of epidermal growth factor, 20 mg of glycerol and 15 mg of yeast extract in 10 ml of phosphate buffer (pH 6.8), shake well and add to a spinner bottle to rotate the hydrated phospholipid film, remove it for ultrasonic treatment in an ice-water bath , sonicate for 50 minutes to fully hydrate the lipid film to obtain epidermal growth factor complex liposome III.
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