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Simple acer palmatum sangokaku tissue culture propagation process

A technology of red maple and chicken feet, which is applied in the field of simple tissue culture propagation technology of maple red maple with chicken feet, can solve the problem that the method of tissue culture propagation of red maple has not been reported, and achieves low production cost, simple formula and easy acquisition. Effect

Active Publication Date: 2014-01-01
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is a relatively common and precious ornamental variety in the market today, but there has never been a report on the tissue culture propagation method of red maple

Method used

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  • Simple acer palmatum sangokaku tissue culture propagation process
  • Simple acer palmatum sangokaku tissue culture propagation process
  • Simple acer palmatum sangokaku tissue culture propagation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The simple tissue culture propagation technique of Acer palmatum and red maple comprises the following steps:

[0022] S1. Sterilize the explants. Collect disease-free branches of Acer palmatum and red maple in the same year at noon or afternoon on a sunny day, remove excess leaves, cut into stems with buds and sterilize them. Sterilization method, first sterilize with alcohol for 30 seconds, then sterilize with mercuric chloride for 5 minutes, and then wash with sterile water for 4 to 6 times;

[0023] S2, inoculation and propagation, inoculate the sterilized stem section with buds on the growth medium, the medium is MS+IAA0.5mg / L, and place the inoculated culture material in a light intensity of 2000-3000LX, The light time is 16h / d, and the temperature is 24-26°C in a cultivation room for 15 days to induce germination of axillary buds, such as figure 1 shown;

[0024] S3, rooting culture, cutting directly induced axillary buds, inoculated on the rooting medium, such...

Embodiment 2

[0026] The simple tissue culture propagation technique of Acer palmatum and red maple comprises the following steps:

[0027] S1. Sterilize the explants. Collect disease-free branches of Acer palmatum and red maple in the same year at noon or afternoon on a sunny day, remove excess leaves, cut into stems with buds and sterilize them. Sterilization method, first sterilize with alcohol for 50 seconds, then sterilize with mercuric chloride for 4 minutes, and then wash with sterile water for 4 to 6 times;

[0028] S2, inoculation and propagation, inoculate the sterilized stem section with buds on the growth medium, the medium is MS+IAA0.5mg / L, and place the inoculated culture material in a light intensity of 2000-3000LX, The light time is 16h / d, and the temperature is 24-26°C in a culture room for 2 weeks to induce germination of axillary buds, such as figure 1 shown;

[0029] S3, rooting culture, cutting directly induced axillary buds, inoculated on the rooting medium, such as ...

Embodiment 3

[0031] The simple tissue culture propagation technique of Acer palmatum and red maple comprises the following steps:

[0032] S1. Sterilize the explants. Collect disease-free branches of Acer palmatum and red maple in the same year at noon or afternoon on a sunny day, remove excess leaves, cut into stems with buds and sterilize them. Sterilization method, first sterilize with alcohol for 60 seconds, then sterilize with mercuric chloride for 3 minutes, and then wash with sterile water for 4 to 6 times;

[0033] S2, inoculation and propagation, inoculate the sterilized stem section with buds on the growth medium, the medium is MS+IAA0.5mg / L, and place the inoculated culture material in a light intensity of 2000-3000LX, The light time is 16h / d, and the temperature is 24-26°C in a culture room for 3 weeks to induce germination of axillary buds, such as figure 1 shown;

[0034] S3, rooting culture, cutting directly induced axillary buds, inoculated on the rooting medium, such as ...

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Abstract

The invention discloses a simple acer palmatum sangokaku tissue culture propagation process. The process comprises the following steps: S1. carrying out explant sterilization: collecting the disease-free acer palmatum sangokaku branches in current year, removing redundant leaves, cutting the branches into stems with buds and sterilizing; S2. carrying out inoculation and propagation: inoculating the sterilized stems with buds onto a culture medium for growth and inducing axillary bud to germinate; and S3. carrying out rooting culture: cutting the directly induced axillary bud and inoculating the axillary bud onto a rooting culture medium to induce root generation. The process has the beneficial effects that the acer palmatum tissue culture sangokaku propagation system is established, the breakthrough of the acer palmatum sangokaku propagation system is realized, simple tissue culture propagation technology is obtained, the technology has no callus stage, the tissue culture seedling can be quickly obtained and can be transplanted and survive rapidly after propagation, the production cost is low, the obtained tissue culture seedlings have consistent hereditary feature, the propagation coefficient is high, the propagation period is short, the process is an efficient path for rapid propagation of the acer palmatum sangokaku and virus accumulation is avoided.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a simple tissue culture propagation technique of Acer palmatum and red maple. Background technique [0002] The genus Acer (Aceraceae) is usually referred to as maple, and it is also called "maple" or "maple" in habit. There are more than 200 kinds of maple trees in the world, distributed in Asia, Europe, North America and northern Africa. There are more than 160 species in my country, accounting for more than 75% of the world's total. They are distributed all over the country, and are mainly produced in the Yangtze River Basin and the provinces to the south. Maple trees can be used as building materials, paper-making raw materials, medicine extraction, industrial oil and nectar plants. Some maple trees are rich in tree shape, elegant in posture, rich in leaf shape and changeable in leaf color, and are known as world-famous foliage plants. Acer palmatum (Acer palm...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 马建华王小辉朱晓菲吴佳川沈香兰
Owner SICHUAN COLORLINK CO LTD
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