Method for detecting sodium valproate content of blood through high performance liquid chromatography

A high-performance liquid chromatography and sodium valproate technology, which is applied in the field of high-performance liquid chromatography for detecting the content of sodium valproate in blood, can solve the problems of low accuracy, high blood drug concentration of sodium valproate and the like, and achieves the accuracy of High, low detection cost, good recognition effect

Inactive Publication Date: 2013-12-18
上海兰卫医学检验所股份有限公司
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  • Application Information

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Problems solved by technology

[0005] In order to overcome the defects of high price and low precision in determining the blood drug concentration of sodium valproate in the prior art, the invention provides a method for high performance liquid chromatography to detect the content of sodium valproate in blood, and the technical scheme is as follows:

Method used

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  • Method for detecting sodium valproate content of blood through high performance liquid chromatography
  • Method for detecting sodium valproate content of blood through high performance liquid chromatography
  • Method for detecting sodium valproate content of blood through high performance liquid chromatography

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Experimental program
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Effect test

Embodiment 1

[0070] Embodiment 1: on the chromatogram with absorbance as the ordinate and time as the abscissa, draw the peak of the cyclohexanecarboxylic acid internal standard solution when the retention time is 5.749 and the sodium valproate in the patient's blood at the retention time of The peak at 10.639, according to Image 6 The standard curve of sodium valproate, the instrument automatically calculates the sodium valproate content in the patient's blood to be 39.23637ng / ul.

Embodiment 2

[0071] Embodiment 2: on the chromatogram with absorbance as the ordinate and time as the abscissa, the peak of the cyclohexanecarboxylic acid internal standard solution when the retention time is 5.743 and the sodium valproate in the patient's blood are at the retention time of The peak at 10.671, according to Image 6 The standard curve of sodium valproate, the instrument automatically calculates the sodium valproate content in the patient's blood to be 64.77381ng / ul.

Embodiment 3

[0072] Embodiment three: the peak when the retention time of cyclohexanecarboxylic acid internal standard solution is 5.752 and the sodium valproate in the patient's blood is obtained on the chromatogram with absorbance as the ordinate and time as the abscissa. The retention time is The peak at 10.658, according to Image 6 The standard curve of sodium valproate, the instrument automatically calculates that the content of sodium valproate in the patient's blood is 118.66935ng / ul.

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Abstract

The invention relates to the technical field of biochemical and provides a method for detecting sodium valproate content of blood through high performance liquid chromatography. The method comprises the steps of preparing 37.6mug / ml of a sodium valproate standard solution, sodium valproate working solutions with various concentrations, 50.2mug / ml of a hexahyl carbonic acid internal standard solution, a blank sample, a normal human blood sample and a patient blood sample; putting into a chromatographic instrument to draw chromatograms, and judging whether the blood of a patient contains sodium valproate according to the chromatograms; then drawing a sodium valproate standard curve with the chromatographic instrument according to the chromatogram of the normal human blood sample, and calculating the sodium valproate content of the blood of the patient according to a peak sodium valproate value of the chromatogram of the patient blood sample, a peak value of the hexahyl carbonic acid internal standard solution and the sodium valproate standard curve. According to the method, the problems that the chromatograms contain multiple stray peaks, the sensitivity is low, the precision is low, the cost is high, and the sodium valproate standard curve cannot be drawn are effectively solved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and particularly relates to a method for detecting the content of sodium valproate in blood by high performance liquid chromatography. Background technique [0002] Sodium valproate is a commonly used broad-spectrum anti-epileptic drug and a drug for the treatment of affective mental disorders, especially for simple absence seizures, generalized rigidity, seizures, and muscle spasms. Sexual seizures have a significant curative effect, and the effective plasma concentration ranges from 50 to 100 μg / ml. However, the in vivo process and efficacy of sodium valproate vary greatly among individuals, the correlation between the dose and the blood drug concentration is unstable, and the high blood drug concentration can easily cause adverse reactions such as nausea, vomiting and liver function damage. Dosage adjustment is a necessary means to ensure efficacy and medication safety. [0003] At prese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 冯娟甄燕
Owner 上海兰卫医学检验所股份有限公司
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