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Applications of TRADD (Tumor necrosis factor receptor associated death domain protein) gene over-expressed lentivirus for inhibiting formation of skin scars

A gene overexpression, lentivirus technology, applied in the field of biopharmaceuticals, can solve the problems of insufficient penetration of laser treatment, long treatment course, and difficult for patients to adhere to.

Inactive Publication Date: 2013-12-18
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the long course of treatment, compression therapy is difficult for patients to adhere to; corticosteroids are only suitable for small areas of pathological scars; the limitations of laser therapy are mainly its insufficient penetration and insufficient depth of treatment; the limiting factors of cryotherapy are pain, delayed healing, and infection

Method used

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  • Applications of TRADD (Tumor necrosis factor receptor associated death domain protein) gene over-expressed lentivirus for inhibiting formation of skin scars
  • Applications of TRADD (Tumor necrosis factor receptor associated death domain protein) gene over-expressed lentivirus for inhibiting formation of skin scars
  • Applications of TRADD (Tumor necrosis factor receptor associated death domain protein) gene over-expressed lentivirus for inhibiting formation of skin scars

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Experimental program
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Effect test

Embodiment Construction

[0028] 1Materials and methods

[0029] 1.1 Plasmids and cells

[0030] TRADD gene template was purchased from OPEN company, lentiviral expression vector pLV X-EGFP-3FLAG-Puro was purchased from Shanghai Shengbo company, ViraPower TM Lenti-viral Packaging Mix was purchased from Invitrogen. DH5a competent cells, 293FT cells, EFb and HSFb are all common cell types in this field.

[0031] 1.2 Main reagents

[0032] DL2000 DNA Marker, agarose gel DNA recovery kit, Taq enzyme and dNTPs were purchased from TaKaRa, EcoRI was purchased from NEB, BCA Protein Assay kit was purchased from HyClone-Perce, Prestained protein marker was purchased from Ferments, Rabbit Anti- TRADD was purchased from Abcam Company, Goat Anti-rabbit IgG was purchased from Santa Cruz Company, ECL-plus / kit was purchased from Amersham Company, and Type I collagen (Collagen I) ELISA kit was purchased from Shanghai Xitang Biotechnology Co., Ltd. The primer synthesis was completed by Shanghai Jierui Biological Engineering ...

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Abstract

The invention discloses applications of TRADD (Tumor necrosis factor receptor associated death domain protein) gene over-expressed lentivirus for inhibiting formation of skin scars or treating skin scars, and particularly discloses a preparation method of the TRADD gene over-expressed lentivirus. The TRADD gene over-expressed lentivirus can simultaneously improve the smoothness and color uniformity of the skin scars.

Description

Technical field [0001] The invention belongs to the field of biopharmaceuticals, and specifically relates to the application of a TRADD gene overexpression lentivirus in inhibiting the formation of skin scars. Background technique [0002] The human TRADD gene contains 4 exons. The total length of the exons is 939bp, which can encode a signal protein of 34KD-tumor necrosis factor receptor associated death domain protein (TRADD). The protein can regulate cell proliferation and apoptosis through a signal pathway mediated by tumor necrosis factor alpha (TNF-α). After TNF-α binds to TNFR1, the death domain (DD) of the intracellular segment of TNFR1 can bind to TRADD protein, resulting in two different induction and regulation mechanisms (1) TRADD binds to FADD and caspase-8, and then Activate caspase-3 to induce cell apoptosis; (2) TRAD combines TRAF (TNF receptor associated factors) or recruits RIP (receptor interacting protein) to activate NF-κB, activated NF-κB can inhibit cell a...

Claims

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Application Information

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IPC IPC(8): A61K35/76C12N15/74A61P17/02C12N7/01
Inventor 彭贵勇袁月康秀峰刘云杰代剑华
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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