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Applications of Sarcaboside A in medicament for treating yellow fever virus infection

A yellow fever virus and drug technology, applied in the field of biomedicine, to achieve huge social benefits and research value

Inactive Publication Date: 2013-12-18
NANJING GUANGKANGXIE BIOLOGICAL MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no cases reported in China, but this does not make us relax our vigilance against the virus, because China's climate and geographical environment are complex, and there are many types of mosquitoes, which have the conditions for transmission. At the same time, with the increasing frequency of international exchanges, YFV It is very likely that it will be introduced into China

Method used

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  • Applications of Sarcaboside A in medicament for treating yellow fever virus infection
  • Applications of Sarcaboside A in medicament for treating yellow fever virus infection
  • Applications of Sarcaboside A in medicament for treating yellow fever virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: the preparation of compound Sarcaboside A tablet involved in the present invention:

[0039] Take 20 grams of compound Sarcaboside A, add 180 grams of conventional excipients for tablet preparation, mix well, and make 1000 tablets with a conventional tablet press.

Embodiment 2

[0040] Embodiment 2: the preparation of compound Sarcaboside A capsule involved in the present invention:

[0041] Get 20 grams of compound Sarcaboside A, add conventional adjuvants for preparing capsules such as 180 grams of starch, mix well, and pack into capsules to make 1000 tablets.

[0042] The following pharmacodynamic experiments will further illustrate its drug activity.

experiment example 1

[0044] A kind of application of Sarcaboside A in preparation treatment or prevention yellow fever virus infection medicine, its steps are:

[0045] A. Toxicity test of Sarcaboside A on Vero cells

[0046] Vero cells (African green monkey kidney cells) are susceptible cells to YFV.

[0047] The experimental steps are as follows:

[0048] 1: Inoculate Vero cells: Use DMEM medium containing 10% (v / v) fetal bovine serum to make a single cell suspension, inoculate 1000-10000 cells per well into a 96-well cell culture plate, and inoculate a volume of 100ul per well ;

[0049] 2: Culture Vero cells: at 37°C, 5% (v / v) CO 2 Under culture conditions, cultivate for 2 days;

[0050] 3: Add Sarcaboside A: Discard the DMEM medium in each well, add 100ul to each well and dilute to the corresponding concentration with DMEM medium containing 10% (v / v) fetal bovine serum (0uM, 0.4uM, 1.2 uM, 3.7uM, 11uM, 33uM, 100uM, 300uM) of Sarcaboside A, add 100ul of DMEM medium containing 10% (v / v) fe...

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Abstract

The invention discloses applications of Sarcaboside A in preparing a medicament for treating or preventing yellow fever virus infection. The Sarcaboside A can effectively inhibit the proliferation of yellow fever virus, and can be further developed into a medicament for treating yellow fever virus infection-caused diseases due to low cell toxicity, thus having wide application prospects. The applications of the Sarcaboside A in a medicament for treating yellow fever virus infection are disclosed for the first time. As a skeleton type of the Sarcaboside A belongs to a brand-new skeleton type, the Sarcaboside A has unexpectedly strong yellow fever virus inhibition activity, and the possibility of giving any inspiration by other compounds does not exist. The Sarcaboside A has significantly substantive characteristics, as well as obviously prominent progress in the prevention and treatment of the yellow fever virus infection.

Description

technical field [0001] The invention belongs to the field of biomedicine, and more specifically relates to the application of Sarcaboside A in the preparation of medicines for treating or preventing yellow fever virus infection. Background technique [0002] Yellow fever virus (YFV) is a member of the Flavivirus genus in the family Flaviviridae. Yellow fever virus causes yellow fever, an acute infectious disease. Yellow fever has been designated as a quarantine infectious disease internationally, and China has also designated it as a Class A infectious disease. In 1648, the YUCATAN peninsula in America first confirmed the prevalence of yellow fever. From the 17th to the 19th century, the disease was brought to Europe and North America through transportation. Within two centuries, yellow fever became one of the most serious plagues in some parts of the United States, Africa, and Europe, causing a large number of deaths. Since the 20th century, the disease has not occurred ...

Claims

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Application Information

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IPC IPC(8): A61K31/365A61P31/14
CPCY02A50/30
Inventor 江春平张广
Owner NANJING GUANGKANGXIE BIOLOGICAL MEDICAL TECH
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