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Growth-promoting cultivation method for fritillaria unibiacteata

A technique of fritillary fritillary and cultivation method, which is applied in the field of rapid propagation of fritillary fritillary, can solve the problems of no breeding system, not many studies of fritillary fritillary, and no scale of reproduction method, so as to shorten the cultivation time. , control of pests and diseases, high utilization rate

Inactive Publication Date: 2013-12-18
北京橘井健康科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the tissue culture technology of Fritillaria has begun to take shape, but there are not many researches on Fritillaria fritillaria, and the reproduction method has not yet been scaled up, and a complete reproduction system has not been formed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A growth-promoting cultivation method for Fritillaria oleifera, comprising:

[0023] (1) Selection and disinfection of materials: Firstly, select vigorous and mature fresh dark purple fritillary bulbs, peel off the outermost dry and wrinkled scales of the bulbs and residual old roots at the base, rinse with running water for 30 minutes, and then put them into clean water Soak for 15 minutes, soak in 50% carbendazim 500 times solution for 20 minutes, peel off the bulbs layer by layer, and then irradiate under the ultraviolet lamp for 20 minutes, then transfer to the ultra-clean workbench, and put them on the ultra-clean workbench Soak in 70% ethanol for 10 seconds, then rinse with sterile water, then transfer to 0.1% mercuric chloride solution with 2 drops of soil temperature of 80°C and soak for 5 minutes, and rinse with sterile water for 4 times;

[0024] (2) Material processing: Blot the sterilized scales dry with sterile filter paper, divide the material into upper, ...

Embodiment 2

[0031] A growth-promoting cultivation method of dark purple Fritillaria, comprising the following steps:

[0032] (1) Selection and disinfection of materials: Firstly, select vigorous and mature fresh dark purple fritillary bulbs, peel off the outermost dry and wrinkled scales of the bulbs and residual old roots at the base, rinse with running water for 30 minutes, and then put them into clean water Soak for 15 minutes, soak in 50% carbendazim 500 times solution for 30 minutes, peel off the bulb layer by layer, and then irradiate under the ultraviolet lamp for 20 minutes, and then transfer to the ultra-clean workbench, in the ultra-clean work The table is soaked with 75% ethanol for 10 seconds, then rinsed with sterile water, and then soaked in 0.1% mercuric chloride solution with 3 drops of soil temperature 80°C for 5 minutes, and rinsed with sterile water for 4 times;

[0033] (2) Material processing: Blot the sterilized scales dry with sterile filter paper, divide the mater...

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Abstract

The invention discloses a growth-promoting cultivation method for fritillaria unibiacteata. The growth-promoting cultivation method comprises the steps of selection and disinfection of materials, treatment of the materials, primary culture, secondary culture, rooting culture, greenhouse acclimatization and transplanting of rooting seedlings in test tubes and soil matrix management. According to the growth-promoting cultivation method, fresh fritillaria unibiacteata bulbs are adopted as explants for propagation, the utilization rate of the bulbs is as high as above 95%, and cultivated plants have flourish branches and leaves and developed root systems, and can vigorously grow. One bulb with the diameter of 4cm can propagate about 150-200 small bulbs, so that limited resources can be utilized for propagation, and the propagation coefficient is very high. In the process of the secondary culture, after thin buds are generated through callus differentiation, the culture temperature is raised, the illumination period is increased, the illumination intensity is enhanced, and the soil fertility and the control rate of diseases and pests are improved, so that the culture time is shortened.

Description

technical field [0001] The invention relates to a method for tissue culture of Fritillaria, in particular to a method for rapid propagation suitable for Fritillaria oleifera. Background technique [0002] Dark Purple Fritillaria (F. unibiacteata Hsiao et K. C. Hsia) is a relatively valuable Chinese medicinal material in the genus Fritillaria. Its bulbs can effectively relieve cough, eliminate phlegm, clear heat and moisten the lungs. It is especially suitable for the elderly and children with persistent deficiency and cold cough. The demand for dark purple Fritillaria in the Chinese medicinal materials market is particularly high, but its bulbs grow very slowly. It usually takes four years to grow from seeds to commercial medicinal materials. The difficulty is high, the reproduction coefficient is low, and the growth cycle is long, so the price of commercial medicinal materials has remained high, and the demand has increased in recent years, resulting in higher and higher p...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G1/00
Inventor 吴志刚
Owner 北京橘井健康科技集团有限公司
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