Culture method for Antrodia

Abstract

The invention provides a culture method for Antrodia. The culture method includes preparing water, 0.1 to 50 wt% of glucose, 1 to 5 wt% of peptone, 0.1 to 50 wt% barley extract, 0.1 to 50wt% yeast extract and 0.5 to 10 wt% of curing agent into cylinder culture medium with the depth ranging from 2 to 20 mm; arranging a plurality of predetermined inoculating positions inside the cylinder culture medium with the depth ranging from 0.5 to 10 mm spacing from the outer surface of the cylinder culture medium, and inoculating the Antrodia to the predetermined inoculating positions; culturing the cylinder culture medium at the temperature ranging from 23 to 28 EDG C for at least three months, and obtaining the Antrodia products. By the aid of the culture method for the Antrodia, growing quantity of Antrodia mycelium or fruiting bodies in one unit can be increased, infectious microbe contamination can be reduced, and production loss can be reduced.

Description

technical field [0001] The invention relates to a method for cultivating Antrodia camphorata, in particular to a method for cultivating Antrodia camphorata that can produce triterpenoids from Antrodia camphorata. Background technique [0002] Antrodia camphorata (Taiwanofungus camphorates), also known as Antrodia camphorata or Antrodia camphorata, belongs to the Basidiomycota, Basidiomycotina, Homobasidiomycetes, and Aphyllophorales of the fungal kingdom. , Polyporaceae, and Antrodia, Antrodia camphorata only grows on Taiwan's protected tree species Cinnamomum camphora, which is a unique mushroom in Taiwan. [0003] From the mycelia or fruiting body extracts of Antrodia Cinnamomea, a variety of physiologically active substances were found, such as polysaccharides, adenosine or a variety of triterpenoids, wherein, among these triterpenoids, such as Amino A (Antcin A), Antcin B (Antcin B), Antcin C (Antcin C), Antcin E (Antcin E), Antcin F (Antcin F), Methylantcinate G (Methy...

AI Technical Summary

Problems solved by technology

[0006] The solid-state culture method in the prior art is to dig out a piece of Antrodia camphorata mycelium or fruiting body about 1 cm square, and attach it to the surface of the solid medium, so that the Antrodia camphorata can be attached to the surface of the solid medium growth, although the mycelium or fruiting body of Antrodia camphorata can be obtained by the solid-state culture method of the prior art, and it is difficult to produce miscellaneous bacteria, yet, only Contains a very low amount of triterpenoids, so the mycelium or fruiting body of Antrodia camphorata obtained by the solid-state culture method of the prior art cannot be applied to extract its physiologically active substances and thus not used in industry

[0007] The liquid fermentation method in the prior art is to inoculate the mycelium of Antrodia Cinnamomea into a liquid culture solution. Although the liquid culture solution can increase the physiologically active substances contained in Antrodia Cinnamomea, it is necessary to add such as bagasse or whole grains during the cultivation process. The solid substrate serves as the support for the mycelium of Antrodia cinnamomea. Therefore, the liquid fermentation method must be cultivated in a large-volume reactor, and its preparation cost is high, and the liquid fermentation method is also prone to contamination by bacteria

[0008] The basswood cultivation method in the prior art is to inoculate Antrodia camphorata on the basswood of Antrodia camphorata, and the obtained Antrodia camphorata components are similar to the types of physiologically active substances contained in wild Antrodia camphorata, but because the basswood of Antrodia camphorata is not easy to obtain, and it is difficult to cultivate The cost is high, so it is impossible to produce triterpenoids in large quantities

[0009] Although the space bag cultivation method of the prior art has a low overall cost, it is easily contaminated by bacteria during the cultivation process, and the content of physiologically active substances is low. Therefore, it is impossible to mass-produce and prepare triterpenoids

[0010] Though above-mentioned method all can obtain the Antrodia camphorata mycelium or the fruiting body containing triterpenoids, its culture operation is not easy, easily produces miscellaneous bacteria, makes the Antrodia camphorata that the whole batch culture is polluted and causes the great loss in the production, and now The quality of mycelium or fruiting body of Antrodia camphorata produced by the cultivation method of Antrodia camphorata with technology is not high, and the triterpenoids contained in it are still insufficient

[0011] Therefore, the Antrodia camphorata cultivation method of the prior art still has deficiencies, and needs to be further improved.

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