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Helicobacter pylori rapid-growth additive and preparation method thereof

A growth additive and a technology for Helicobacter pylori, applied in the field of Helicobacter pylori rapid growth additive and preparation thereof, can solve problems such as poor growth of weak bacteria, and achieve the effects of shortening the era time and improving the detection positive rate

Active Publication Date: 2013-12-11
杭州致远医学检验所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0029] Bacteria have an adaptation time on the medium, that is, the lag period ((0~a), generally takes 2 hours, and generally takes 20 minutes to reproduce for one generation after entering the logarithmic growth phase, and at the same time, some weak and small bacteria have the problem of poor growth

Method used

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  • Helicobacter pylori rapid-growth additive and preparation method thereof
  • Helicobacter pylori rapid-growth additive and preparation method thereof
  • Helicobacter pylori rapid-growth additive and preparation method thereof

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Embodiment 1

[0059] One, the preparation of Helicobacter pylori rapid growth additive:

[0060](1) Take 500g of pig stomach and 500g of beef as raw materials, rinse them with tap water, remove tendons, membranes, and grease, then soak and rinse with tap water in turn, then filter out the water, dry, and cut into 1 cm 3 The size of small pieces, the raw materials are required to be bright in color, fresh, no peculiar smell, no oil, freshly obtained, if not used in time, it can be stored in an environment of 4-8 °C for no more than 8 hours to achieve freshness, generally 4 ~8 hours;

[0061] (2) Put the raw material cut into small pieces in step (1) into a large bucket, add purified water twice the quality of the raw material to mix, heat and boil for 1 hour, filter with gauze, and collect the filtrate A;

[0062] (3) Put the filter residue obtained in step (2) in a large bucket, add pure water whose mass is double that of the raw material, mix, heat and boil for 1 hour, filter with gauz...

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Abstract

The invention discloses a helicobacter pylori rapid-growth additive. The rapid-growth additive is prepared by the following components in parts by mass: 500 parts of pig stomachs, 500 parts of beef, 7 parts of sodium chloride, 1 part of insoluble starch, 1 part of urea, and right amount pure water and sodium bicarbonate; the pH value is adjusted to be 7.4 to 7.6; after pretreatment, stripping and slicing, boiling for two times, filtering, filter liquor mixing and cold storage, boiling again, cooling, filtering, constant volume, sodium chloride addition, insoluble starch and urea dissolution, pH adjustment, sterilization and storage are performed on raw materials sequentially, the rapid-growth additive is prepared. The rapid-growth additive provided by the invention has the advantages that the lag phase of indicator bacteria is promoted to be shortened when the indicator bacteria are cultured in vitro, the growth and reproduction time of the indicator bacteria on culture medium is shortened effectively, the detection positive rate is improved, the rapid-growth additive is used for preparing high-quality helicobacter pylori isolation medium, and the rapid-growth additive can be prepared through the preparation method disclosed by the invention.

Description

[0001] technical field [0002] The invention relates to a rapid-growth additive, in particular to a Helicobacter pylori rapid-growth additive for preparing a high-quality Helicobacter pylori isolation medium and a preparation method thereof. Background technique [0003] The growth and reproduction of general bacteria has the following characteristics: [0004] 1. Individual growth and reproduction of bacteria [0005] Bacteria usually reproduce by simple asexual binary fission, and very few bacteria can have branched division. Under suitable conditions, most bacteria reproduce very quickly, and it only takes 20-30 minutes to divide once; a few bacteria multiply slowly, such as Mycobacterium tuberculosis, which divides once in about 18-20 hours. [0006] The colony growth and reproduction of bacteria [0007] Bacteria reproduce very quickly, and generally bacteria divide once every 20 minutes, that is, one generation. Calculated at this speed, after 10 hours, one bacter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 杨宁敏屠苗颖
Owner 杭州致远医学检验所有限公司
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