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Preparation method of silkworm ecdysterone

A technology of ecdysterone and temperature control, applied in the field of ecdysterone preparation, can solve the problems of inconvenient storage, low purity of silkworm ecdysis liquid, unable to produce and use quantitative standards, etc. neat effect

Inactive Publication Date: 2013-12-11
SICHUAN LONGHAIYANG ANIMAL PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention overcomes the disadvantages of low purity of silkworm molting liquid produced in the prior art, cannot make quantitative standards for use, and is inconvenient for storage, and provides an embodiment of a preparation method of silkworm ecdysterone, in order to improve the efficiency of silkworm molting. The purity of the liquid, making it possible to make quantitative standards for use and easy to store

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 5kg of Herba sinensis, crush it, then add 50L of water to stir and cook, the temperature is controlled at about 90°C, then use a multi-functional extraction tank to reflux for extraction for 1 hour, filter while it is hot, save the filtrate for later use, and continue the rough extraction of the filter residue Twice, the method is the same as the above-mentioned crude extraction method, except that the water added to the medicinal residues is 25 L each time, and the filter residue is extracted twice and discarded, and the filtrate obtained from the above-mentioned three rough extractions is collected for use. Control the temperature of the filtrate collected during the rough extraction at about 70°C, add 5L of 1% ZTCl+1 1% acetic acid solution to the filtrate, stir once every 30 minutes, filter after two hours, collect the filtrate, continue Keep the temperature around 70°C, add 2.5L of 5% ZTCl+1 aqueous solution to the filtrate, stir once every 30 minutes, filter a...

Embodiment 2

[0030] Take 2kg of Herba sinensis, crush it, then add 20L of water to stir and cook, the temperature is controlled at about 80°C, then use a multi-functional extraction tank to reflux extract for 45 minutes, filter while it is hot, save the filtrate for later use, and continue the rough extraction of the filter residue Twice, the method is the same as the above-mentioned crude extraction method, except that the water added to the medicinal residues is 10L each time, and the filter residue is extracted twice and discarded, and the filtrate obtained from the above-mentioned three rough extractions is collected for use. Control the temperature of the filtrate collected during the rough extraction at about 60°C, add 2L of 1% ZTCl+1 1% acetic acid solution to the filtrate, stir once every 25 minutes, filter after 100 minutes, collect the filtrate, continue Keep the temperature around 60°C, add 1L of 5% ZTCl+1 aqueous solution to the filtrate, stir once every 25 minutes, filter after...

Embodiment 3

[0032]Take 4kg of Herba sinensis, crush it, then add 40L of water to stir and cook, the temperature is controlled at about 100°C, then use a multi-functional extraction tank to reflux extract for 50 minutes, filter while it is hot, save the filtrate for later use, and continue the rough extraction of the filter residue Twice, the method is the same as the above-mentioned crude extraction method, except that the water added to the medicinal residues is 20L each time, and the filter residue is extracted twice and discarded, and the filtrate obtained from the above-mentioned three rough extractions is collected for use. Control the temperature of the filtrate collected during the rough extraction at about 80°C, add 4L of 1% ZTCl+1 1% acetic acid solution to the filtrate, stir once every 27 minutes, filter after 110 minutes, collect the filtrate, continue Keep the temperature around 80°C, add 2L of 5% ZTCl+1 aqueous solution to the filtrate, stir once every 27 minutes, filter after...

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Abstract

The invention discloses a preparation method of silkworm ecdysterone. The preparation method comprises the following steps of: boiling, refluxing and extracting decumbent bugle herb; clarifying the collected filtrate by using a clarificant and then performing reduced pressure concentration to obtain a solid residue; dissolving the solid residue by using a methanol-ethyl acetate solution; performing reduced pressure concentration to remove an organic solvent and then obtaining a crude product of an ecdysterone extract; separating and purifying the ecdysterone by using macroporous resin and a silica-gel chromatographic column; and finally performing vacuum concentration to obtain an ecdysterone dry product. The ecdysial fluid prepared from the ecdysterone dry product prepared by the preparation method is high in purity and can be used in a quantified mode, the ecdysterone dry product is convenient to store, the storage time is long, and by adopting the ecdysial fluid prepared from the ecdysterone dry product, domestic silkworms grow and develop neatly and are mounted neatly.

Description

technical field [0001] Embodiments of the present invention relate to the field of processing and manufacturing traditional Chinese veterinary medicines, and more specifically, embodiments of the present invention relate to a preparation method of ecdysterone for preparing molt liquid for silkworms. Background technique [0002] The manufacturing process of existing molt liquid for silkworms is a traditional production process, and its preparation method is "get 5000g of Herba sinensis, cut into small sections, add water and decoct twice, each time for 1 hour, filter and remove the medicinal residues, merge the filtrate, reduce Concentrate under pressure to about 8000ml, add 25000ml of ethanol, let stand for 24 hours after stirring, filter, recover ethanol from the filtrate under reduced pressure, concentrate the remaining liquid to about 5000ml, add 20000ml of ethanol, stir, let stand for 12 hours, filter, and filtrate Recover ethanol under reduced pressure, concentrate the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00
Inventor 姚书兰
Owner SICHUAN LONGHAIYANG ANIMAL PHARMA CO LTD
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