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Method for preparing quinolinone alkaloid and application of quinolinone alkaloid used as environmentally-friendly marine organism antifouling compound

An alkaloid and quinolinone technology, which is applied in the preparation of quinolinone alkaloids and the application field as an environment-friendly marine biological antifouling agent, can solve problems such as marine biological antifouling agents that have not yet been seen

Active Publication Date: 2013-11-27
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, no marine biological antifouling agent with important application value has been obtained from marine microorganisms

Method used

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  • Method for preparing quinolinone alkaloid and application of quinolinone alkaloid used as environmentally-friendly marine organism antifouling compound
  • Method for preparing quinolinone alkaloid and application of quinolinone alkaloid used as environmentally-friendly marine organism antifouling compound
  • Method for preparing quinolinone alkaloid and application of quinolinone alkaloid used as environmentally-friendly marine organism antifouling compound

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Embodiment Construction

[0008] Preparation of Compounds of the Invention

[0009] (1) The strain medium used for the strain culture of fungus Aspergillus sp. (HM991281) contains glucose 1.0% (weight percentage, the same below), yeast extract 0.1%, peptone 0.2%, agar 1.0%, coarse sea salt 3%, The rest is water, which is made into a test tube slant when used, and the fungal strains are cultivated at 28°C for 5 days.

[0010] The strain culture medium contains 0.1%-5.0% of glucose (percentage by weight, the same below), 0.01%-2% of yeast extract, 0.01%-2% of peptone, 0.1%-3.0% of agar, and 0.05%-5% of coarse sea salt. %, the rest is water; the culture temperature is 5-45°C; the culture time is 3-10 days.

[0011] (2) The fermentation medium used for the fermentation culture of the fungus Aspergillus sp. (HM991281) contains glucose 1.0% (weight percentage, the same below), yeast extract 0.1%, peptone 0.2%, coarse sea salt 3.0%, and the rest is water; The strains were cultured at 25°C for 30 days.

[0...

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Abstract

The invention provides a method for preparing quinolinone alkaloid and an application of quinolinone alkaloid used as an environmentally-friendly marine organism antifouling compound. The method comprises the following steps of: performing strain culture on marine fungus Aspergillus sp. (HM991281) in a strain culture medium; performing fermentation culture on the fungus in a fermenting culture medium, and filtering the fermentation liquid; removing thallus, concentrating filtrate, and extracting with ethyl acetate; decompressing the extract, performing chromatographic separation, and concentrating eluent to obtain a colorless oily object which is aflaquinolone D. The environmentally-friendly marine organism antifouling compound has the characteristics that the quinolinone alkaloid is used as an effective component and used for preventing marine organisms from being fouled due to Balanus amphitrilte larva, raw materials can be produced in large scale through fungus fermentation and is not limited by resources, so that the method has a wide application prospect.

Description

technical field [0001] The invention relates to a preparation method of a quinolinone alkaloid with the activity of inhibiting the attachment of barnacle larvae and its application as an environment-friendly marine biological antifouling agent. Background technique [0002] Marine biofouling means that the larvae of marine fouling organisms such as barnacles, mussels, and bryozoans attach and grow on the surface of marine facilities such as hulls, fish tanks, oil wells, or other marine organisms to form communities, thereby causing huge damage to the attached objects. Damage, such as accelerating metal corrosion, reducing the performance of marine facilities, affecting the output and quality of aquaculture, etc. Among marine fouling organisms, barnacles are the most widely distributed and most harmful. The economic loss caused by marine biofouling is huge every year all over the world. Taking ships as an example, fouling organisms adhere to the bottom of the ship, which in...

Claims

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Application Information

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IPC IPC(8): C07D215/22A01N43/42A01P17/00C09D5/16C12P17/12C12R1/66
Inventor 王长云邵长伦陈敏
Owner OCEAN UNIV OF CHINA
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