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Grafting and seed multiplication method for endangered tobacco genetic resources

A germplasm and resource technology, applied in botany equipment and methods, horticulture, application, etc., can solve problems such as poor control effect of pesticides, troublesome operation, and generation of drug resistance, so as to reduce field mortality, save costs, and prevent seedlings. The effect of mass loss

Inactive Publication Date: 2013-11-27
YUNNAN ACAD OF TOBACCO AGRI SCI
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AI Technical Summary

Problems solved by technology

However, the disadvantage of potted plant propagation in raw red soil is that new red soil must be replaced every time the seed is propagated, which is cumbersome to operate; in addition, is the method of potted plant propagation in raw red soil suitable for the propagation of germplasm resources with high susceptibility to bacterial wilt? unknown
[0003] Tobacco black shank and bacterial wilt are soil-borne rhizome diseases, which have brought harm to tobacco production for a long time, causing huge losses in production and economic income, and are also a major problem in tobacco seed propagation
Some methods of preventing and treating black shank with chemical agents are disclosed in Chinese patent documents, such as 200710065691.3, 200710300087.4, 200810233500.4, etc. However, in practical applications, the occurrence of black shank varies with varieties, and sometimes the control effect of chemical agents is not good. even drug resistance
Chemical control is also less effective against bacterial wilt

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] 1. Preliminary determination of endangered tobacco germplasm resources: Through a comprehensive cleaning of the seeds of the tobacco gene bank, the list of various tobacco germplasm resources with a very small amount of seed storage (<3g) is initially determined as endangered tobacco species qualitative resources;

[0018] 2. Formal determination of endangered tobacco germplasm resources: compare the preliminary list of endangered tobacco germplasm resources to check the seed collection records over the years, as well as the field test data and seed reproduction of various germplasm resources in each year, and further list more years. The list of tobacco germplasm resources (endangered tobacco germplasm resources) whose seeds were not reproduced due to root diseases such as black shank and bacterial wilt died during seed propagation in the second field, excluding those with less seed storage due to frequent seed picking Germplasm resources that are easily propagated in ...

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Abstract

The invention relates to a grafting and seed multiplication method for saving endangered tobacco genetic resources and belongs to agriculture seed multiplication technologies and the field of genetic resources seed multiplication. The seed multiplication method includes the steps of carrying out seed cleaning on a tobacco genetic bank, listing various tobacco genetic resources with seed storage amount smaller than 3g, screening out the genetic resources which are high in death rate because of infection of root stock diseases like blackleg and bacterial wilt in seed multiplication processes over the years, adopting varieties resistant to the tobacco root stock diseases as stocks, adopting susceptible genetic resources as cions for grafting, receiving seeds of cion varieties and storing the seeds. The grafting and seed multiplication method is simple, capital and manual investment is little, the genetic resources can be effectively prevented from becoming extinct and bad influences and damage to the environment cannot be caused.

Description

technical field [0001] The invention relates to a grafting and multiplication method for saving endangered tobacco germplasm resources, which belongs to the fields of agricultural seed multiplication technology and germplasm resources multiplication. Background technique [0002] Tobacco germplasm resources contain extremely rich genetic genes and biodiversity, which is the key to preventing the occurrence of diseases and ensuring the sustainable development of tobacco agriculture, as well as the material basis for tobacco breeding and biotechnology research. In 2005, when Yunnan Tobacco Genebank was cleaned up, it was found that the seed storage capacity of some germplasm resources was less than 10g, and some seed storage capacity was even less than 3g. Since 2006, seed propagation and storage work has been arranged in the field year by year to supplement the seed storage capacity of the gene bank and ensure the continuous supply and utilization of germ plasm resources. Ho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/06
Inventor 许美玲肖炳光刘勇曾建敏焦芳婵吴兴富
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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