Fruit extract mixture-containing external-agent composition for skins
A skin external preparation, fruit extraction technology, applied in the field of natural product chemistry
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Embodiment 1
[0038] Embodiment 1: the preparation of fruit extract
[0039] Longan fruit dried and pulverized powder 50g, cold soaked with 500ml of 70% ethanol at room temperature, ultrasonically extracted twice, each time 30min (500W), the filtrates were combined, and the solvent was recovered under reduced pressure to dryness to obtain the longan extract.
[0040] Mangosteen fruit dried and pulverized powder 50g, cold soaked with 500ml of 70% ethanol at room temperature, ultrasonically extracted twice, each time for 30min (500W), the filtrates were combined, and the solvent was recovered under reduced pressure to dryness to obtain the mangosteen extract.
[0041] Carambola fruit dried and pulverized powder 50g, under room temperature with 70% ethanol 500ml cold soaking, ultrasonic extraction 2 times, each 30min (500W), filtrate is combined, decompression recovery solvent is dry, obtains carambola extract.
Embodiment 2
[0042] Example 2: Preparation of a mixture of fruit extracts
[0043] Longan extract and mangosteen extract were mixed according to weight ratios of 10:1, 5:1, 1:1, 1:5 and 1:10 respectively, and prepared into a solution for corresponding efficacy test.
[0044] Longan extract and carambola extract were mixed according to weight ratios of 10:1, 5:1, 1:1, 1:5 and 1:10 respectively, and prepared into solutions for corresponding efficacy tests.
[0045] Mangosteen extract and carambola extract were mixed according to weight ratios of 10:1, 5:1, 1:1, 1:5 and 1:10 respectively, and prepared into solutions for corresponding efficacy tests.
[0046] Take longan extract, mangosteen extract and carambola extract, and mix them according to the weight ratio of 10:1:1, 5:1:1, 1:1:1, 1:5:5 and 1:10:10 respectively , prepared into a solution for the corresponding efficacy test.
Embodiment 3
[0047] Example 3: Research on anti-aging effects of fruit extract combinations
[0048] 1) Research on free radical scavenging
[0049] Principle: According to the DPPH free radical has a single electron, there is a strong absorption at 517nm, and its alcohol solution is purple. When there is a free radical scavenger, the absorption gradually disappears due to the pairing with its single electron, and the degree of fading is similar to that of The number of accepted electrons has a quantitative relationship, so a rapid quantitative analysis can be performed with a spectrophotometer to detect the free radical scavenging situation, so as to judge whether the sample has antioxidant capacity. Its ability is expressed by clearance rate, and the greater the clearance rate, the stronger its antioxidant capacity.
[0050] Test method: Add 2ml of the sample solution to be tested with different dilutions and different proportions into the test tube, then add 2ml of 76μmol / LDPPH ethanol...
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