Leaf in-vitro culturing method for miniature ornamental photinia fraseri

Abstract

A leaf in-vitro culturing method for miniature ornamental photinia fraseri comprises: employing an annual tender leaf of perennial miniature ornamental photinia fraseri as an explant, after disinfection and sterilization, inoculating into a callus induction culture medium, placing a dark place for 10-15 days, placing under a common fluorescent lamp, at 20-25 DEG C illuminating for 15 hours every day with humidity of 50%-60% for 48 days, to induct and form a callus; cutting the callus and transplanting to a disinfected differentiation propagation medium for propagation culture; after continuous propagation, cutting the propagated test-tube seedling and transplanting to a disinfected seedling-strengthening culture medium for seedling-strengthening culture; after 30 days removing the basal callus and parts of leaves, leaving 3-4 sheets of leaves, inoculating to a disinfected rooting culture medium for rooting culture for 8-10 days; and taking out and washing, transplanting to a substrate containing peat and yellow subsoil with a volume ratio of 3:2, wherein rooting surviving is realized after 15 days and the rooting rate reaches 96% or more.

Description

technical field [0001] The invention relates to a method for rapid propagation of plant culture tissue, in particular to a tissue culture method in which miniature ornamental Photinia fragrans leaves are used as explants. Background technique [0002] Miniature ornamental Photinia lochengensis Yü is an evergreen shrub or small tree. It has advantages: 1) It has good cold resistance, can withstand the lowest temperature of -20°C, can grow normally in the south of Beijing, and has better cold resistance than other Photinia varieties; The new leaves are all bright red and bright red, and the autumn leaves germinate in late September and turn bright red again. Compared with other Photinia fruticosa varieties, the red leaf period is longer, the color is bright, the branches and leaves are dense and small, and the germination ability is strong. Strong branches, good ornamental effect; 3) Moderate growth rate, but slower than other varieties, can be widely used in color blocks and...

AI Technical Summary

Problems solved by technology

In the existing reproduction technology, the method of tissue culture has been reported, but in the report, the buds of the shoots that were born in the same year are used as the buds of the explants to carry out the tissue culture and propagation, and the use of explants is many, and the proliferation cycle is longer. Problems such as low multiplication coefficient and low reproduction efficiency