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High performance liquid chromatography method for determination of lumefantrine content

A technology of high performance liquid chromatography and benzfluorenol, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that cannot meet the requirements of sample analysis and quality control, and achieve the effect of good injection precision

Inactive Publication Date: 2013-10-16
ZHANG JIA GANG VINSCE BIO PHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its standard collection and Chinese Pharmacopoeia 2010 edition two, its content determination method is titration method, which is not enough to meet the requirements of sample analysis and quality control, and the method of high performance liquid chromatography determination of lumefantrine content is rarely reported

Method used

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  • High performance liquid chromatography method for determination of lumefantrine content
  • High performance liquid chromatography method for determination of lumefantrine content
  • High performance liquid chromatography method for determination of lumefantrine content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] High performance liquid chromatography method-1 for the determination of benzfluorenol content

[0017] Chromatographic conditions: US Agilent-1260 high performance liquid chromatograph, Chemstation Edition chromatographic workstation

[0018] Chromatographic column: Thermo BDS-C18 (150mm×4.6mm, 5μm)

[0019] Mobile phase: methanol: water: glacial acetic acid = 80: 20: 1 (volume ratio) (pH = 4)

[0020] Detection temperature: 30°C

[0021] Detector: UV detector

[0022] Detection wavelength: 264nm

[0023] Vacuum pump: SHB-III circulating water vacuum pump

[0024] Electronic balance: provided by Mettler-Toledo Instruments (Shanghai) Co., Ltd., the specification is one hundred thousandth

[0025] Preparation of the mobile phase: The mobile phase was mixed according to methanol: water: glacial acetic acid = 80: 20: 1 (volume ratio) (pH = 4), filtered through a filter with a pore size of 0.2 μm, and degassed by SCQ-250W ultrasonic wave.

[0026] Using the external s...

Embodiment 2

[0034] High performance liquid chromatography method for the determination of benzefantrine content-2

[0035] Chromatographic conditions: US LabAlliance HPLC, EZChrom Elite Client chromatographic workstation

[0036] Chromatographic column: SMT-SAM-C18 (250mm×4.6mm, 5μm)

[0037] Mobile phase: methanol: water: glacial acetic acid = 80: 20: 1 (volume ratio) (pH = 4)

[0038] Detection temperature: 30°C

[0039] Detector: SPECTRA STSTEM, UV6000LP ultraviolet detector

[0040] Detection wavelength: 264nm

[0041] Vacuum pump: SHB-III circulating water vacuum pump

[0042] Electronic balance: provided by Mettler-Toledo Instruments (Shanghai) Co., Ltd., the specification is one hundred thousandth

[0043] Preparation of the mobile phase: The mobile phase was mixed according to methanol: water: glacial acetic acid = 80: 20: 1 (volume ratio) (pH = 4), filtered through a filter with a pore size of 0.2 μm, and degassed by SCQ-250W ultrasonic wave.

[0044] Using the external sta...

Embodiment 3

[0052] High performance liquid chromatography method-3 for the determination of benzfluorenol content

[0053] Chromatographic conditions: US Agilent-1260 high performance liquid chromatograph, Chemstation Edition chromatographic workstation

[0054] Chromatographic column: Thermo BDS-C18 (250mm×4.6mm, 5μm)

[0055] Mobile phase: methanol: water: glacial acetic acid = 80: 20: 1 (volume ratio) (pH = 4)

[0056] Detection temperature: 30°C

[0057] Detector: UV detector

[0058] Detection wavelength: 264nm

[0059] Vacuum pump: SHB-III circulating water vacuum pump

[0060] Electronic balance: provided by Mettler-Toledo Instruments (Shanghai) Co., Ltd., the specification is one hundred thousandth

[0061] Preparation of the mobile phase: The mobile phase was mixed according to methanol: water: glacial acetic acid = 80: 20: 1 (volume ratio) (pH = 4)), filtered through a 0.2 μm pore size filter, and degassed by SCQ-250W ultrasonic wave.

[0062] Using the external standard m...

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Abstract

The invention discloses a high performance liquid chromatography method for determination of lumefantrine content. Specifically, the chromatographic column is: Thermo BDS-C18(250mm*4.6mm, 5micrometers); the mobile phase is composed of: methanol, water and glacial acetic acid in a volume ratio of 80:20:1 (pH=4); the detection temperature is 30DEG C; the sample size is 5 microliters; the flow rate is 1.0ml / min; the detector is an ultraviolet detector; the detection wavelength is 264nm. Lumefantrine shown a good linear relationship with a peak area in a concentration range of 0.5 microgram / ml-50 microgram / ml, and the linear correlation coefficient r is 0.9998. The high performance liquid chromatography method for detecting the lumefantrine content has the technical characteristics of high sensitivity, accuracy and reliability, good separation effect, simple and fast analysis and the like.

Description

technical field [0001] The invention relates to a high-performance liquid chromatography method for determining the content of benzfluorenol. Background technique [0002] Malaria is caused by Plasmodium and transmitted by mosquitoes. It is the most morbid and harmful mosquito-borne infectious disease in the world today. It mainly occurs in the tropical areas of central Africa, South Asia, Southeast Asia and northern South America. [0003] Since the discovery of Plasmodium falciparum resistance to the antimalarial drug chloroquine in the 1960s, drug-resistant Plasmodium falciparum has spread across all endemic regions of Plasmodium falciparum so far, and the resistance intensity has continued to increase. The speed of Plasmodium resistance is much faster than the speed of new drug research, which greatly limits the clinical application of traditional antimalarial drugs and brings new difficulties to malaria prevention and control. In order to protect new drugs and prolong ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 彭学东张梅赵金召杨艳丽
Owner ZHANG JIA GANG VINSCE BIO PHARM
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