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Method of using biotransformation of corncobs and rapeseed cakes to prepare composite enzyme and probiotics preparation for forage

A technology of rapeseed cake and probiotic preparations, which is applied in animal feed, animal feed, application, etc., can solve the problems of difficult digestion of monogastric animals, and achieve industrialized large-scale production, easy growth of microorganisms, and no waste water discharge Effect

Active Publication Date: 2013-10-09
JINHUA ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, while the cake is rich in protein, it also contains a relatively high amount of plant fiber, which is not easy for monogastric animals to digest.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Fully mix 10kg of fermented Pu-erh cooked tea with 10kg of corncobs, 5kg of rapeseed cake, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water, and cultivate them at 35°C for 48 hours to form a starter;

[0027] 2) Fully mix 20kg of the starter in step 1) with 600kg of corncobs, 300kg of rapeseed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm and natural ventilation at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0028] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 78.83IU / g; total cellulase activity reaches 6.27IU / g; beta-glucosidase activity reaches 6.27IU / g; reached 14.82IU / g; endoglucanase activity reached 58.34IU / g; xylanase activity reached 78.45IU / g; beta-xylosidase activity reached 5.06IU / g; polyga...

Embodiment 2

[0030] 1) Fully mix 10kg of fermented Pu-erh cooked tea with 10kg of corncobs, 5kg of rapeseed meal, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and cultivate them at 35°C for 48 hours to form a starter;

[0031] 2) Fully mix 20kg of the starter in step 1) with 600kg of corncobs, 300kg of rapeseed cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm and natural ventilation at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0032] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 78.75IU / g; total cellulase activity reaches 6.34IU / g; beta-glucosidase activity reaches 78.75IU / g; reached 14.69IU / g; endoglucanase activity reached 58.37IU / g; xylanase activity reached 78.53IU / g; beta-xylosidase activity reached 5.14IU / g; polyg...

Embodiment 3

[0034] 1) Fully mix 10kg of fermented Pu-erh cooked tea with 10kg of corncobs, 5kg of rapeseed cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and cultivate them at 40°C for 36 hours to form a starter;

[0035] 2) Fully mix 10kg of starter in step 1) with 600kg of corncobs, 300kg of rapeseed cake, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm, naturally ventilated, and fermented at 35°C After 5 days, a compound enzyme and probiotic preparation for feed was obtained.

[0036] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 70.45IU / g; total cellulase activity reaches 6.23IU / g; beta-glucosidase activity reaches 70.45IU / g; reached 13.96IU / g; endoglucanase activity reached 60.42IU / g; xylanase activity reached 71.24IU / g; beta-xylosidase activity reached 4.88IU / g;...

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Abstract

A disclosed method of using biotransformation of corncobs and rapeseed cakes to prepare a composite enzyme and a probiotics preparation for a forage comprises the following steps: fully mixing Pu'er cooked tea after pile fermentation, first corncobs, first rapeseed cakes, first wheat bran, first apple pomaces and first water, domestication culturing and forming a starter; and fully mixing the starter, second corncobs, second rapeseed cakes, second wheat bran, second apple pomaces and second water, and fermenting to obtain the composite enzyme and the probiotics preparation for the forage. The composite enzyme and the probiotics preparation for the forage with various better performance indexes, is prepared by using a microecology-stable foodstuff-grade-safe flora to ferment the corncobs and the rapeseed cakes, and the low-cost high-efficiency biotransformation of the corncobs and the rapeseed cakes is realized. At the same time, the method of the invention has the advantages that the microbes are easy to grow, enzyme vitality is high, the fermentation process is extensive, no strict aseptic condition is needed, and the industrialized large-scale production is easy to realize.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for preparing a compound enzyme and probiotic preparation for feed by fermenting corncob and rapeseed meal with microecologically stable food-grade safe flora. Background technique [0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed materials, especially energy feed and protein feed. [0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large number of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban fibrous waste, by-products of agricultural product processing such as corn cob, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulose, hemicellulose, ligni...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14
Inventor 阮晖项云徐志钱春芳陈功诸文颖纪晓燚吴渊杜姗姗杨璐
Owner JINHUA ACAD OF AGRI SCI
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