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Method for extracting and preparing peroxidase from stem and leaves of sweet potato

A technology for peroxidase and sweet potato, which is applied in the field of protein preparation and extraction, can solve the problems of resource waste, low utilization rate of nutrients, environmental pollution, etc., and achieves the effects of low production cost, low equipment investment and low energy consumption.

Inactive Publication Date: 2013-10-02
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these dosages are relatively small, and the utilization rate of nutrients is low.
At present, most of the sweet potato stems and leaves are discarded as waste, resulting in a large waste of resources and environmental pollution

Method used

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  • Method for extracting and preparing peroxidase from stem and leaves of sweet potato
  • Method for extracting and preparing peroxidase from stem and leaves of sweet potato
  • Method for extracting and preparing peroxidase from stem and leaves of sweet potato

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Such as figure 1 Shown in the flow chart of the present invention, the concrete steps of the method that the present invention extracts and prepares peroxidase from the stem and the leaf of sweet potato are as follows:

[0030] (1) Prepare 100g of fresh sweet potato stems and / or leaves;

[0031] (2) Homogenize the stem and / or leaves of the sweet potato with a phosphate buffer with a solid-to-liquid ratio of 1:1, extract for 1 hour, centrifuge or filter to obtain a crude peroxidase extract ;

[0032] (3) Add solid ammonium sulfate to the peroxidase crude extract to a saturation concentration of 40% to 90%, or add ethanol or acetone to a final concentration of 50% to 80%, and after precipitation for 1 hour, separate Supernatant and precipitate, the precipitate is peroxidase;

[0033] (4) Freeze-drying the peroxidase obtained in the step (3) to obtain high-activity peroxidase enzyme powder;

[0034] (5) Recover the ethanol or acetone in the supernatant of the step (3) ...

Embodiment 2

[0037] The peroxidase crude extract obtained in step (2) in the embodiment 1, the peroxidase precipitate obtained in step (3) and the peroxidase enzyme powder obtained in step (4) were respectively analyzed by spectrophotometry The peroxidase enzyme activity and the separation and purification effect were detected by the method and non-denaturing polyacrylamide electrophoresis gel autographic method, and the results are as follows:

[0038] Please refer to figure 2 , extract peroxidase from the different organs of sweet potato with the method of the present invention, comprise the root tuber of sweet potato, new stem and old stem and new leaf and old leaf, the result shows, from the old stem and leaf of sweet potato (no matter new and old), especially the content of active peroxidase extracted and prepared from the old stem is the highest, thus determining the most suitable part of sweet potato for extracting peroxidase.

[0039] Please refer to Figure 3-5 , the "stock sol...

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Abstract

The invention discloses a method for extracting and preparing peroxidase from the stems and leaves of sweet potato. The method comprises the following steps of: performing homogenate extraction on the stems and / or leaves of sweet potato by use of water or a phosphate buffer solution; centrifuging or filtering to obtain protein crude enzyme extraction liquid; precipitating the extraction liquid by use of ammonium sulfate or an organic solvent to obtain separated and purified peroxidase; and drying to obtain high-activity peroxidase powder. The method disclosed by the invention further develops the sweet potato and turns waste into wealth, is energy-saving and environment-friendly and is simple to operate; and the production cost of the product is low, and large-scale production is facilitated.

Description

technical field [0001] The invention relates to a protein preparation and extraction technology, in particular to a method for extracting and preparing peroxidase from sweet potato stems and leaves. Background technique [0002] According to the statistics of the Food and Agriculture Organization of the United Nations, the planting area of ​​sweet potatoes in my country has reached 3.68 million ha 2 , accounting for about 60% of the world's total sweet potato area, with a total output of 81 million tons, accounting for about 80% of the world's total output. Due to the extensive cultivation of sweet potatoes, a large number of by-products - sweet potato stems and leaves - are produced. my country produces 70 billion kilograms of sweet potato stems and 72 billion kilograms of sweet potato leaves annually. [0003] The stems and leaves of sweet potatoes contain a lot of nutrients, the crude protein content is as high as 16.5-17.1%, which is higher than the content of rice and...

Claims

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Application Information

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IPC IPC(8): C12N9/08
Inventor 李海航林佳刘洁倪贺
Owner SOUTH CHINA NORMAL UNIVERSITY
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