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Highly-active humic acid microbial inoculant and preparation method thereof

A technology of highly active humic acid and microbial inoculum, which is applied in the directions of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem of low biological activity of humic acid, inoculant function is not obvious, and effective bacteria content is low. and other problems, to achieve the effects of improving the root environment, excellent resistance to continuous cropping, and a large number of beneficial bacteria

Inactive Publication Date: 2013-10-02
内蒙古永业生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation technology of these humic acid microbial agents is single, the biological activity of humic acid is not high, the functionality of the agent is not obvious, and the content of effective bacteria is low. It is necessary to further develop microbial agents with stronger applicability and more outstanding effects.

Method used

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  • Highly-active humic acid microbial inoculant and preparation method thereof
  • Highly-active humic acid microbial inoculant and preparation method thereof
  • Highly-active humic acid microbial inoculant and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] A kind of highly active humic acid biological bacteria agent and preparation method thereof, specifically as follows:

[0045] Step 1: Prepare endogenous inhibitory bacteria:

[0046] (1) Isolation, screening and identification of endogenous inhibitory bacteria

[0047] A. Separation

[0048] Select a soil sample from a cucumber growing healthy area in a plot with severe powdery mildew disease of cucumber, dilute the sample mass by 30 times with sterile water, shake evenly, put it in water at 90°C for 15 minutes, and dilute it gradually to 10~, take 0.1 mL was inoculated onto the beef extract peptone agar medium plate and spread evenly. Place it in an incubator at 25°C for dark culture for 3 days, and after a single colony grows, purify, transfer to a tube, and store;

[0049] B. Screening

[0050] Put the material obtained in A ascomycete powdery mildew (Sphaerotheca fuliginea) in the center of the PDA plate, inoculate the inhibitory bacteria to be tested around th...

Embodiment 2

[0076] A kind of highly active humic acid biological bacteria agent and preparation method thereof, specifically as follows:

[0077] Step 1: Prepare endogenous inhibitory bacteria:

[0078] (1) Isolation, screening and identification of endogenous inhibitory bacteria

[0079] A. Separation

[0080] Take soil samples from cucumber powdery mildew severe disease areas and select healthy areas of cucumber growth. After diluting 10 times the mass of the sample with sterile water, shake it evenly, put it in water at 80°C for 20 minutes, and dilute it to 10-4 gradiently. Inoculate 0.1mL onto the beef extract peptone agar medium plate and spread evenly. Place in a 30°C incubator for dark culture for 4 days. After a single colony grows, purify, transfer, and store;

[0081] B. Screening

[0082] Put the material obtained in A ascomycete powdery mildew (Sphaerotheca fuliginea) in the center of the PDA plate, inoculate the inhibitory bacteria to be tested around the plate, and inocu...

Embodiment 3

[0108] A kind of highly active humic acid biological bacteria agent and preparation method thereof, specifically as follows:

[0109] Step 1: Prepare endogenous inhibitory bacteria:

[0110] (1) Isolation, screening and identification of endogenous inhibitory bacteria

[0111] A. Separation

[0112] Take soil samples from cucumber powdery mildew seriously diseased plots and select cucumber growing healthy areas, dilute the sample mass parts by 20 times with sterile water, shake evenly, put them in water at 85°C for 18 minutes, and gradually dilute to 10-4, take Inoculate 0.1mL onto the beef extract peptone agar medium plate and spread evenly. Place it in an incubator at 28°C for dark culture for 3 days. After a single colony grows, purify, transfer, and store;

[0113] B. Screening

[0114] Put the material obtained in A ascomycete powdery mildew (Sphaerotheca fuliginea) in the center of the PDA plate, inoculate the inhibitory bacteria to be tested around the plate, and in...

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Abstract

The invention relates to a highly-active humic acid microbial inoculant. The highly-active humic acid microbial inoculant comprises 0.2-5% of an inoculant, 95-99.8% of an auxiliary material, and 0.01-0.3% of an activator; the inoculant comprises 50-70% of an endotrophic inhibition bacterium and 30-50% of a beneficial synergistic bacterium; and the auxiliary material comprises 50-80% of an organic material and 20-40% of active humic acid. The microbial inoculant has the characteristics of reasonable compatibility of florae, large quantity of beneficial bacteria of a product, high biological activity, stable effects, and lasting fertilizer efficiency, has disease control and growth promotion effects, can be used for producing green and organic vegetables as a typical good product, can improve the soil fertility when applied to soil, degrades pesticide residual in soil, inhibits soil borne diseases, performs a replant resistance, promotes the absorption of plants to nutritional elements, improves the fertilizer utilization rate, enhances the immunities of the plants, enhances the stress resistances of the crops, promotes the root development, and finally improves the output and quality of the crops.

Description

Technical field: [0001] The invention relates to a highly active humic acid microbial bacterial agent fertilizer prepared by using mineral humic acid, in particular to a highly active humic acid microbial bacterial agent and its preparation. Background technique: [0002] my country is a large agricultural country, and the amount of chemical fertilizer application ranks first in the world. At present, agriculture pursues the yield per unit area, and the excessive use of chemical fertilizers leads to a downward trend in the utilization rate of chemical fertilizers year by year, resulting in the destruction of soil structure, the decline of organic matter content, and the stability of natural biodiversity and ecosystems. There are a series of serious problems such as the reduction of agricultural production, the eutrophication of rivers and lakes, and the decline in the quality of agricultural products. Gradually reduce the amount of pesticides and chemical fertilizers, and gra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C05G3/00C12R1/56C12R1/125C12R1/07C12R1/865C12R1/245
Inventor 高觅王琦仝宝生吴子申周涛张雪花袁红利
Owner 内蒙古永业生物技术有限责任公司
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