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P-hydroxy phenylalanine urine detection reagent and preparation method thereof

A technology of hydroxyphenylalanine and detection reagent, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem that the reliability of statistical results is not strong and is not applicable. Tumor screening, small number of specimens, etc., to achieve the effect of quick response, obvious results, and simple operation

Inactive Publication Date: 2014-07-09
吉林生物研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The reagents provided by the above-mentioned prior art have certain effects in application, high specificity and sensitivity, but there are still deficiencies. The reagents provided by Chinese patent CN1096374 have a high false positive rate, greater than 10%, and are not applicable Due to the general screening of tumors, the number of EP341803 specimens tested is small, so the reliability of the statistical results is not strong

Method used

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  • P-hydroxy phenylalanine urine detection reagent and preparation method thereof
  • P-hydroxy phenylalanine urine detection reagent and preparation method thereof
  • P-hydroxy phenylalanine urine detection reagent and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0054] ①. Preparation of liquid A: Add 18g of mercury sulfate into 6mol / L sulfuric acid solution and stir to dissolve, then dilute to 100ml with 6mol / L sulfuric acid solution to obtain a colorless and transparent liquid for use;

[0055] ②. Preparation of liquid B: add 65g of mercurous nitrate into 12mol / L nitric acid solution, dissolve and dilute to 100ml with 12mol / L nitric acid solution to obtain a green liquid, which can be used after the green color of the solution fades;

[0056] ③. Preparation of liquid C: Dissolve 3 g of nickel sulfate in distilled water, then dilute to 100 ml with distilled water to obtain a blue-green liquid for use;

[0057] ④, preparation of liquid D: dissolve 2g of α-nitroso-β-naphthol in 50ml, 6mol / L nitric acid solution to obtain liquid D for use;

[0058] ⑤. Mixing: Place A, B, C, D, and four kinds of liquids prepared in the above ①, ②, ③, and ④ in a container according to the volume ratio of 10ml:12ml:2ml:0.5ml and mix them to obtain the targe...

Embodiment 2

[0060] A, B, C, D, the preparation of four kinds of liquids are the same as embodiment 1, after A, B, C, D, four kinds of liquids are placed in the container by volume ratio 12ml:15ml:3ml:0.7ml and mix, namely get the target product.

Embodiment 3

[0062] A, B, C, D, the preparation of four kinds of liquids are the same as in Example 1, after A, B, C, D, four kinds of liquids are placed in the vessel and mixed by volume ratio 15ml:18ml:4ml:1ml, promptly get target product.

[0063] In actual production, it is enough to expand according to the ratio of the above-mentioned embodiment.

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Abstract

The invention relates to an in-vitro detection reagent for clinical diagnosis, particularly a reagent for determining metabolic disorder in a human body, particularly a p-hydroxy phenylalanine urine detection reagent and a preparation method thereof. The urine detection reagent is prepared by respectively dissolving Hg2<+>, Hg<2+>, Ni<2+> and alpha-nitroso-beta-naphthol in corresponding acid solutions and proportionally mixing. The mercuric sulfate is dissolved in a sulfuric acid solution as a donor of Hg<2+>, the mercurous nitrate is dissolved in a nitric acid solution as a donor of Hg2<+>, the nickel sulfate is dissolved in distilled water as a donor of Ni<2+>, and the alpha-nitroso-beta-naphthol is dissolved in a nitric acid solution. The invention overcomes the defects in the prior art, and provides a new-formula metabolic disorder quick urinalysis reagent which has the advantages of high stability, high sensitivity and wide application range.

Description

technical field [0001] The invention relates to an in vitro detection reagent for clinical diagnosis, in particular to a reagent for judging abnormal metabolism in the human body—a urine detection reagent for p-hydroxyphenylalanine and a preparation method thereof. Background technique [0002] Major metabolic diseases such as malignant tumors and diabetes caused by abnormal cell metabolism in the body have attracted more and more attention. The key to treating diseases caused by abnormal metabolism in the body lies in early detection, early diagnosis and early treatment. At present, the resulting disease diagnosis methods mainly include physics, histocytology and biochemistry. For tumor diseases, physics and histocytology often can only detect the middle and late stages, and it is difficult to achieve the purpose of early detection, and most of the operations are complicated and expensive. [0003] Early diagnosis of metabolic diseases such as malignant tumors is the most ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/82
Inventor 张凤华
Owner 吉林生物研究院有限公司
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