Method for reproducing magnolia zenii seedlings
A technology for Baohua magnolia and seedlings, which is applied in the field of Baohua magnolia seedling propagation, can solve the problems of difficulty in applying Baohua magnolia to large-scale breeding, high cost and large labor consumption of Baohua magnolia, and achieves the improvement of cutting survival rate, Low cost and long rooting time
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Embodiment 1
[0024] In June 2011, researchers conducted related experiments on cuttings at Nanjing Forestry University, and selected (1) factor A of Baohua magnolia branches, three levels: A1 lignified branches in the previous year; A2 semi-lignified branches in the current year; A3 young and tender in the current year branch. ⑵Factor B of the type of rooting agent, three levels: B1.ABT1 (No. ABT1 rooting powder is generally used for cutting seedlings of difficult-to-root plants and precious plants, developed by Professor Wang Tao, an academician of the Chinese Academy of Engineering), B2.NAA (naphthalene acetic acid), B3.IBA (indolebutyric acid). (3) rooting agent concentration C factor, three levels: C1.400㎎ / L, C2.600㎎ / L, C3.800㎎ / L). An orthogonal experiment with three factors and three levels was carried out. The substrate is one third each of perlite, vermiculite and peat.
[0025] The test results showed that: in the cutting test of Baohua Magnolia, the treatment of semi-lignified ...
Embodiment 2
[0027] In June 2012, the researchers conducted another experiment on cuttings in Jurong City Forest Farm under Baohua Mountain in Baohua Magnolia Reserve. 1:1, ④ perlite: peat = 1: 1, ⑤ perlite: vermiculite = 3: 1, ⑥ perlite: peat = 3: 1, ⑦ perlite: vermiculite: peat = 1: 1: 1) ; ⑵Concentration of rooting agent (①400㎎ / L, ②600㎎ / L, ③800㎎ / L); ⑶Treatment time (①10s, ②10min, ③1h), conduct cutting test. The cuttings are semi-lignified branches of the current year, and the rooting agent is an aqueous solution of NAA rooting agent.
[0028] The test results show that: in the 30-day investigation of the cuttage test of Baohua Magnolia, the substrate ③ perlite: vermiculite = 1: 1, the rooting agent concentration ② 600㎎ / L, the treatment time ① 10s are the best, and the rooting rate is 62.22% , callus formation rate (not rooted) 17.78%, root effect index 1.05.
Embodiment 3
[0030] Utilize the method of the present invention to Baohua Magnolia seedling propagation, comprise the following steps:
[0031] (1) In June, when the spring shoots of Baohua Magnolia stop growing and the branches are semi-lignified, cut 8-10 cm semi-lignified branches of Baohua Magnolia with top leaves, and cut off half of the top leaves;
[0032] (2) The lower part of the Baohua Magnolia branch in step (1) is immersed in the rooting agent solution; the rooting agent solution is a NAA rooting agent solution, and the NAA rooting agent powder is first dissolved in alcohol with a concentration of more than 75%, and then formulated into The concentration is 500-700㎎ / LNAA rooting agent aqueous solution;
[0033] (3) Insert the Baohua Magnolia branches treated in step (2) into the cutting matrix for 5-15 seconds, spray the cutting matrix with thiophanate liquid to prevent infection and necrosis of cuttings wounds, and cuttages with Baohua Magnolia branches The cutting substrate ...
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