Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lagerstroemia plant chromosomal in-situ hybridization method

An in situ hybridization and chromosome technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of difficult identification of chromosomes, difficult dyeing, blanks, etc., and achieve high hybridization specificity

Inactive Publication Date: 2013-09-18
BEIJING FORESTRY UNIVERSITY
View PDF3 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the plants in this genus have small and medium-sized chromosomes, and they are not easy to stain. It is difficult to identify their chromosomes by classical cytological methods, which limits the in-depth understanding of the molecular cytogenetic mechanism of Lagerstroemia indica
So far, there is no report on the application of in situ hybridization technology in Lagerstroemia genus, and the research on its genome structure and molecular cytogenetics is still blank.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lagerstroemia plant chromosomal in-situ hybridization method
  • Lagerstroemia plant chromosomal in-situ hybridization method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] 1. Preparation of chromosome slide specimens:

[0050] At 9:00 in the morning, take the stem tip part of the innermost length of the shoot tip growth point of the annual branch of Lagerstroemia urophyllum with a length of 0.5 cm. Put them directly into the fixative solution (by volume, absolute ethanol: glacial acetic acid (analytical grade): chloroform (analytical grade) = 5:3:2) and fix in a -20°C refrigerator for 18 hours. Rinse and wash the fixative in distilled water, transfer to 0.075mol / L KCl solution and hypotonic for 30 minutes; rinse the growth point of the shoot tip after the pre-hypotonic treatment with distilled water, dry it with absorbent paper, and put it into the mixed enzyme solution Enzyme hydrolysis in a water bath at 37°C for 4 hours, and the mixed enzyme solution was prepared by 2.5% cellulase: 2.5% pectinase: 0.01% proteinase K in a volume ratio of 2:1:3. The slides were prepared by the flame drying method, and the microscopic examination was carri...

Embodiment 2

[0066] The difference from Example 1 is that the material is Yakushima crape myrtle. The steps and signal observation are the same as in Example 1.

[0067] The results show that from figure 1 and figure 2 As can be seen in , there are 2 signal points (marked by arrows) in the hybridization of the Dig-labeled maize 45S rDNA probe to the Lagerstroemia genus chromosome preparation. The background interference is weak, and the sensitivity and accuracy are high, so that the in situ hybridization technique can be better applied to the chromosome detection of small chromosome plants such as Lagerstroemia indica, and provides a new way and method for the in-depth study of the chromosomes of Lagerstroemia genus plants.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a lagerstroemia plant chromosomal in-situ hybridization method. The method comprises seven steps such as sheet making, probe preparing, pretreating before hybridization, hybridization solution preparing, probe denaturating, chromosome denaturating, hybridizing and detecting. A lagerstroemia plant metaphase chromosome serves as a target deoxyribose nucleic acid (DNA) and maize 45SrDNA as a probe, and the maize 45SrDNA probe is clearly located on the lagerstroemia plant metaphase chromosome. By utilizing the hybridization method, background interferences can be effectively eliminated, the detection sensitivity and accuracy are improved, the hybridization method can be better applied to the chromosome detection of small chromosome plants such as lagerstroemia and the like, and a new way and a method are provided for the further research of lagerstroemia plant chromosomes.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to an in situ hybridization method for chromosomes of Lagerstroemia genus plants. Background technique [0002] Molecular in situ hybridization (In situ hybridization, referred to as ISH) uses DNA fragments labeled with biotin and other markers as probes to perform molecular hybridization with chromosomal DNA, and directly detect DNA fragments complementary to the probe under a microscope place of existence. The physical positioning of rDNA on chromosomes by fluorescence in situ hybridization technology can not only provide a stable and effective cytologically identifiable chromosome marker for karyotype analysis, but also study the evolutionary relationship between plant species and clarify the structural variation of chromosomes and other important genetic issues. At present, rDNA has been extensively physically located in the genomes of important model crops and commercial c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
Inventor 潘会堂杨冰洁张启翔蔡明程堂仁王佳孙明陈晶鑫
Owner BEIJING FORESTRY UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com